Construct: ORF TRCN0000465446
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009475.1_s317c1
- Derived from:
- ccsbBroadEn_05489
- DNA Barcode:
- TTGACAGCCTACGGCAATACTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRAMEF9 (343070)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465446
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 653619 | PRAMEF15 | PRAME family member 15 | NM_001098376.3 | 100% | 100% | |
| 2 | human | 400735 | PRAMEF4 | PRAME family member 4 | NM_001009611.4 | 98.7% | 98.1% | (many diffs) |
| 3 | human | 343070 | PRAMEF9 | PRAME family member 9 | NM_001010890.2 | 98.3% | 97.4% | (many diffs) |
| 4 | human | 343068 | PRAMEF5 | PRAME family member 5 | NM_001013407.2 | 98.3% | 97.2% | (many diffs) |
| 5 | human | 645359 | PRAMEF26 | PRAME family member 26 | NM_001306072.1 | 98.2% | 97.2% | (many diffs) |
| 6 | human | 441873 | PRAMEF25 | PRAME family member 25 | NM_001310134.1 | 98.2% | 97.2% | (many diffs) |
| 7 | human | 440561 | PRAMEF6 | PRAME family member 6 | NM_001010889.2 | 98.2% | 97% | (many diffs) |
| 8 | human | 101929983 | PRAMEF27 | PRAME family member 27 | NM_001300891.1 | 98.1% | 97% | (many diffs) |
| 9 | human | 440560 | PRAMEF11 | PRAME family member 11 | NM_001146344.3 | 97.9% | 97% | (many diffs) |
| 10 | human | 440560 | PRAMEF11 | PRAME family member 11 | XM_011541479.2 | 97.9% | 97% | (many diffs) |
| 11 | human | 645425 | PRAMEF20 | PRAME family member 20 | NM_001099852.2 | 90.5% | 81.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1503
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagatgagc atccggactc cacccagact cctggagctt gcagggcgga 121 gcctgctgag ggaccaagct ttggccatgt ccaccctgga ggagctgccc acagaacttt 181 tccccccact gttcatggag gccttcagca ggagacgctg tgaggccctg aagctgatgg 241 tgcaggcctg gcccttccgc cgcctccctc tgaggcctct gataaagatg ccttgtctgg 301 aggccttcca agctgtgctc gatgggcttg atgcactgct tacccaaggg gttcgtccca 361 ggaggtggaa actccaagtg ctggatttac aggatgtctg tgagaacttc tggatggttt 421 ggtctgaagc tatggcccat gggtgcttcc tcaatgccaa gaggaacaaa aaaccagtgc 481 aggactgtcc aaggatgaga ggacggcagc ccttgactgt gttcgtagaa ctttggctca 541 agaacaggac tctggatgaa tacctcacct acctccttct atgggtcaag cagaggaaag 601 atttactaca cctgtgctgt aagaagctga aaattttggg aatgcccttc cgcaatatca 661 gaagcatcct gaaaatggtg aacctagact gtatccagga ggtggaagtg aattgcaagt 721 gggtactgcc catcctgaca cagtttaccc catacctggg ccacatgagg aatcttcaga 781 agctcgttct ctcccacatg gatgtctctc gctacgtttc cccagagcag aagaaggaga 841 ttgttaccca gttcaccact cagttcctca agctgcgctg cctccaaaag ctttatatga 901 actctgtttc tttcctcgaa ggccacctgg accagctgct cagctgtctg aagacctcgt 961 taaaagtcct cacaataact aactgtgtgc ttttggaatc agacttgaag catctatccc 1021 agtgcccgag tatcagtcaa ctaaagaccc tggacctgag tggcatcaga ctgaccaatt 1081 atagtcttgt gcctctccaa attctcctag aaaaagttgc agccaccctt gagtacctgg 1141 atttagatga ctgtggcatc atagactccc aagtcaacgc catcctgcct gcccTGAGCC 1201 GCTGCTTTGA GCTCAACACC TTCAGCTTCT GTGGAAATCC CATCTGCATG GCCACCCTGG 1261 AGAACCTGCT GAGCCACACA ATCATACTCA AAAACTTATG TGTGGAGCTG TATCCTGCCC 1321 CCCGAGAGAG TTATGGTGCT GATGGTACTC TCTGCTGGAG CAGATTTGCT CAAATTAGGG 1381 CTGAGCTGAT GAACAGAGTG AGGGACTTAA GGCACCCCAA GAGGATCTTG TTCTGTACTG 1441 ACTACTGCCC TGACTGTGGC AACAGGTCAT TTTATGACCT GGAGGCAGAT CAATACTGCT 1501 GTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1561 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1621 GGCTTTATAT ATCTTGTGGA AAGGACGATT GACAGCCTAC GGCAATACTG TCACGCGTTA 1681 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt