Transcript: Human NM_001098376.3

Homo sapiens PRAME family member 15 (PRAMEF15), mRNA.

Source:
NCBI, updated 2019-06-30
Taxon:
Homo sapiens (human)
Gene:
PRAMEF15 (653619)
Length:
1865
CDS:
95..1531

Additional Resources:

NCBI RefSeq record:
NM_001098376.3
NBCI Gene record:
PRAMEF15 (653619)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001098376.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000282226 CATCAGACTGACCAATTATAG pLKO_005 1090 CDS 100% 13.200 7.920 N PRAMEF15 n/a
2 TRCN0000246044 GCATCAGACTGACCAATTATA pLKO_005 1089 CDS 100% 15.000 7.500 Y PRAMEF9 n/a
3 TRCN0000246041 CCCGAGTATCAGTCAACTAAA pLKO_005 1051 CDS 100% 13.200 6.600 Y PRAMEF9 n/a
4 TRCN0000337185 CCCTTGAGTACCTGGATTTAG pLKO_005 1152 CDS 100% 13.200 6.600 Y PRAMEF11 n/a
5 TRCN0000246089 CTGTCTGAAGACCTCGTTAAA pLKO_005 970 CDS 100% 13.200 6.600 Y PRAMEF5 n/a
6 TRCN0000253801 GAACAGGACTCTGGATGAATA pLKO_005 568 CDS 100% 13.200 6.600 Y PRAMEF20 n/a
7 TRCN0000254726 GGAATGCCCTTCCGCAATATC pLKO_005 665 CDS 100% 13.200 6.600 Y PRAMEF6 n/a
8 TRCN0000246040 GGAGCACAGAACCCATCATTT pLKO_005 1617 3UTR 100% 13.200 6.600 Y PRAMEF9 n/a
9 TRCN0000246043 GGGAATGCCCTTCCGCAATAT pLKO_005 664 CDS 100% 13.200 6.600 Y PRAMEF9 n/a
10 TRCN0000262399 TCTGGACACTTGGAAACTAAA pLKO_005 1570 3UTR 100% 13.200 6.600 Y PRAMEF15 n/a
11 TRCN0000246042 TGACCTGGAGGCAGATCAATA pLKO_005 1501 CDS 100% 13.200 6.600 Y PRAMEF9 n/a
12 TRCN0000254724 TGGCATCAGACTGACCAATTA pLKO_005 1087 CDS 100% 13.200 6.600 Y PRAMEF6 n/a
13 TRCN0000246091 TGTTGAATGCCTGCCTATTTG pLKO_005 1526 CDS 100% 13.200 6.600 Y PRAMEF5 n/a
14 TRCN0000265615 CCGAGTATCAGTCAACTAAAG pLKO_005 1052 CDS 100% 10.800 5.400 Y PRAMEF6 n/a
15 TRCN0000282227 GACCTGGAGGCAGATCAATAC pLKO_005 1502 CDS 100% 10.800 5.400 Y PRAMEF15 n/a
16 TRCN0000262401 GCTGTCTGAAGACCTCGTTAA pLKO_005 969 CDS 100% 10.800 5.400 Y PRAMEF15 n/a
17 TRCN0000246092 TGCTGAGCCACACAATCATAC pLKO_005 1293 CDS 100% 10.800 5.400 Y PRAMEF5 n/a
18 TRCN0000262400 TGGGAATGCCCTTCCGCAATA pLKO_005 663 CDS 100% 10.800 5.400 Y PRAMEF15 n/a
19 TRCN0000337113 ATGGGTATGTCAAACGCTTTC pLKO_005 1548 3UTR 100% 6.000 3.000 Y PRAMEF11 n/a
20 TRCN0000167074 CGAGTATCAGTCAACTAAAGA pLKO.1 1053 CDS 100% 5.625 2.813 Y PRAMEF9 n/a
21 TRCN0000135112 CTCAATGCCAAGAGGAACAAA pLKO.1 476 CDS 100% 5.625 2.813 Y PRAMEF4 n/a
22 TRCN0000172316 CCCTTGACTGTGTTCGTAGAA pLKO.1 536 CDS 100% 4.950 2.475 Y PRAMEF9 n/a
23 TRCN0000135524 CCTCTCCAAATTCTCCTAGAA pLKO.1 1118 CDS 100% 4.950 2.475 Y PRAMEF4 n/a
24 TRCN0000135781 CGTAGAACTTTGGCTCAAGAA pLKO.1 550 CDS 100% 4.950 2.475 Y PRAMEF4 n/a
25 TRCN0000136416 CTCTGAAAGTGGGAAAGGAAA pLKO.1 1648 3UTR 100% 4.950 2.475 Y PRAMEF4 n/a
26 TRCN0000134814 GAACCCATCATTTCACACATA pLKO.1 1625 3UTR 100% 4.950 2.475 Y PRAMEF4 n/a
27 TRCN0000168676 GTGAGAACTTCTGGATGGTTT pLKO.1 426 CDS 100% 4.950 2.475 Y PRAMEF9 n/a
28 TRCN0000135740 CCTCTGATAAAGATGCCTTGT pLKO.1 302 CDS 100% 4.050 2.025 Y PRAMEF4 n/a
29 TRCN0000135479 GAAGGAGATTGTTACCCAGTT pLKO.1 859 CDS 100% 4.050 2.025 Y PRAMEF4 n/a
30 TRCN0000135478 GATGGGTATGTCAAACGCTTT pLKO.1 1547 3UTR 100% 4.050 2.025 Y PRAMEF4 n/a
31 TRCN0000172984 CCGCAATATCAGAAGCATCCT pLKO.1 676 CDS 100% 2.640 1.320 Y PRAMEF6 n/a
32 TRCN0000137305 GCTGGAGCAGATTTGCTCAAA pLKO.1 1380 CDS 100% 0.495 0.248 Y PRAMEF4 n/a
33 TRCN0000172475 CTTCCTCAATGCCAAGAGGAA pLKO.1 472 CDS 100% 0.264 0.132 Y PRAMEF6 n/a
34 TRCN0000337186 GGCATCAGACTGACCAATTAC pLKO_005 1088 CDS 100% 13.200 6.600 Y PRAMEF11 n/a
35 TRCN0000254725 TGTCTGAAGACCTCGTTAAAG pLKO_005 971 CDS 100% 13.200 6.600 Y PRAMEF6 n/a
36 TRCN0000246088 AGGTCTTAGGTACATCCTATA pLKO_005 1595 3UTR 100% 10.800 5.400 Y PRAMEF5 n/a
37 TRCN0000246090 TGACCTGGAGGCAGATCAATG pLKO_005 1501 CDS 100% 10.800 5.400 Y PRAMEF5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001098376.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05489 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05489 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000465446 TTGACAGCCTACGGCAATACTGTC pLX_317 21.8% 100% 100% V5 n/a
4 ccsbBroadEn_10042 pDONR223 100% 98.3% 97.4% None (many diffs) n/a
5 ccsbBroad304_10042 pLX_304 0% 98.3% 97.4% V5 (many diffs) n/a
6 TRCN0000480613 TAAGAGGAAGGCTTACTTACGGTG pLX_317 29.7% 98.3% 97.4% V5 (many diffs) n/a
7 ccsbBroadEn_10041 pDONR223 100% 98.3% 97.2% None (many diffs) n/a
8 ccsbBroad304_10041 pLX_304 0% 98.3% 97.2% V5 (many diffs) n/a
9 TRCN0000465984 CACCCTATCGTCGAGGTCGACTGC pLX_317 21.9% 98.3% 97.2% V5 (many diffs) n/a
10 ccsbBroadEn_10164 pDONR223 100% 98.1% 96.6% None (many diffs) n/a
11 ccsbBroad304_10164 pLX_304 0% 98.1% 96.6% V5 (many diffs) n/a
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