Construct: ORF TRCN0000465453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011942.1_s317c1
- Derived from:
- ccsbBroadEn_05207
- DNA Barcode:
- TGACGAGAATAAGATGAGGTGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf74 (203076)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203076 | C8orf74 | chromosome 8 open reading f... | NM_001040032.2 | 99.8% | 99.6% | 43C>T |
| 2 | human | 203076 | C8orf74 | chromosome 8 open reading f... | XM_024447091.1 | 85.9% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 951
- ORF length:
- 882
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcactctta acaccccagg gagtgaaaga agtcttccaa tttcagagac 121 cacaaggtcg ggagcgcctg cggaggcttc tgaactggga ggagtttgac gaacagagag 181 actcccggag gagcatcctg ctggacaccc tctacgagag catcatcttt gcagtgggca 241 aaggcttccc atgggtggag gtggcccagg tggtcaagtt cacagaagag ctgctaaggg 301 aaaccaaagg ctgctccatt actgaggctg tgacgatcct ggggaacaag cttagagatt 361 accggggcca tttcaacacc acccacctgc tggccctctg tgactacttc caccacacct 421 tcatccgcca ctacaaactc taccagtatg tcctgggcca ggaccagcag gtcgacctga 481 ccgttgccca cctggaggtg tgcatgccac cccatcccct cccgctggcc gagggcatgg 541 acagggactt gtggatccac gagcagcagg tggccacact gacggaggcc gaggcacaga 601 agcgcgccga cgtgctgctc ctgaaagagg cgctgcgcct ggagcgggag aactcgctgc 661 agaaggcgtt cgctgccgcc gcgcctgcgc agcccggcca ggtcctggag agacaggagt 721 tggagagcct catctgccag gcagtccaca cccagatgga gctcctgcag gagctgcTGC 781 AGCGCCAGAT CCAGAACACA TTCGCCATCT TGGACCTGAA GCTTCAGAAG AAGACTCTGA 841 ACCTCAACGC CCCCACCCCT ATCCCGCCCC CCATCACCAG CCACGCAGGC CAGGAGGAAG 901 CCCTGAAGCC CCAAAGAGCG AGCAAAGGAA AGAAAGCGAA GGCAAGGAAG TTGCCAACTT 961 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1021 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1081 CTTGTGGAAA GGACGATGAC GAGAATAAGA TGAGGTGATC ACGCGTTAAG TCgacaatca 1141 acctctggat tacaaaattt gtgaaagatt