Construct: ORF TRCN0000465469
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009838.1_s317c1
- Derived from:
- ccsbBroadEn_11841
- DNA Barcode:
- CCTGACATCCTCTCCATTGTTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PKD2L2 (27039)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465469
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | NM_001258449.2 | 99.9% | 99.8% | 1217T>C |
| 2 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_024446027.1 | 99.9% | 99.8% | 1217T>C |
| 3 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | NM_001300921.2 | 83.7% | 83.6% | 1146_1448del;1520T>C |
| 4 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_024446025.1 | 83.7% | 83.6% | 1146_1448del;1520T>C |
| 5 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | NM_001258448.2 | 80.2% | 80.1% | 974_975ins66;1080_1382del;1454T>C |
| 6 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_024446026.1 | 80.2% | 80.1% | 974_975ins66;1080_1382del;1454T>C |
| 7 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | NM_014386.4 | 80.1% | 78.4% | (many diffs) |
| 8 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_011543318.3 | 78.3% | 78% | 31_32ins102;1044_1346del;1418T>C |
| 9 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_017009343.2 | 76% | 74.6% | (many diffs) |
| 10 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_011543321.2 | 74.1% | 74% | 0_1ins180;966_1268del;1340T>C |
| 11 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_017009344.2 | 74.1% | 74% | 0_1ins180;966_1268del;1340T>C |
| 12 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_017009345.2 | 71.9% | 71.7% | 524_525ins222;924_1226del;1298T>C |
| 13 | human | 27039 | PKD2L2 | polycystin 2 like 2, transi... | XM_024446028.1 | 63.6% | 63.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1635
- ORF length:
- 1569
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaggcgtca cggtggcacc gaggcggggc ttcgaaacat aagttgcatt 121 acagaaagga agtagaaatt acaaccacac ttcaggaatt gttactctac tttatttttt 181 taataaacct atgtatattg acttttggga tggtaaaccc acatatgtat tacttaaaca 241 aggttatgtc atctctattt ttggacactt ctgtgcctgg tgaagaaaga accaacttta 301 agtccattcg cagcataact gatttttgga agtttatgga aggacccctt ttggaaggtc 361 tgtactggga ttcatggtac aataaccagc agctgtataa tttaaagaac agcagtcgca 421 tctactatga aaatatactt ctaggagttc ccagagttcg tcaactaaaa gtccgcaaca 481 acacatgcaa agtctattca tcttttcagt ctttgatgag tgaatgttat ggcaaatata 541 cttctgcaaa tgaagacctc tctaattttg gccttcaaat taatactgaa tggagatatt 601 ctacttctaa taccaactcc ccttggcact ggggatttct tggtgtttac cgaaatgggg 661 gatacatttt cactttatca aaatcgaaat ctgaaaccaa aaacaagttc attgaccttc 721 gactgaacag ctggatcaca agagggacta gagttatttt tattgatttt tccttatata 781 atgctaatgt aaatctattt tgtattatca gattggtggc agaattccct gcaactggag 841 gaatacttac ttcatggcag ttttactctg tgaagctcct cagatatgtt agctactatg 901 actattttat tgcttcctgt gaaatcacat tctgtatttt tctttttgtc ttcacaacac 961 aagaagtcaa aaaaataaaa gaatttaagt ctgcctattt caaaagtatt tggaactggc 1021 tagaattgct acttttgctg ttgtgttttg tggctgtttc cttcaacaca tactataatg 1081 tacaaatttt tctcttactt ggacagctgt tgaaaagtac tgaaaaatat tcagatttct 1141 attttcttgc atgctggcac atttattaca ataatataat tgctattacc atcttttttg 1201 catggataaa gaatatgttc ttggcaatta ttaatgatac ctatTCTGAA GTGAAAGCTG 1261 ACTATTCAAT AGGCAGAAGG CCAGATTTTG AACTTGGCAA AATGATTAAA CAGAGTTACA 1321 AAAATGTTCT CGAGAAATTC AGACTGAAGA AAGCTCAAAA AGATGAAGAC AAGAAAACCA 1381 AAGGCAGCGG AGATTTGGCT GAACAAGCCA GAAGAGAAGG CTTTGACGAA AATGAGATTC 1441 AAAACGCAGA GCAGATGAAA AAATGGAAAG AGAGGCTTGA GAAAAAGTAT TATTCTATGG 1501 AAATTCAAGA TGACTACCAG CCTGTCACTC AAGAAGAATT TCGAGAACTT TTTTTATATG 1561 CTGTGGAGCT GGAGAAGGAA TTACACTACA TCAATTTGAA GCTAAATCAA GTGGTGAGAA 1621 AGGTTTCAGC TCTATACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1681 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1741 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCTGACATCC TCTCCATTGT 1801 TAGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt