Construct: ORF TRCN0000465478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001586.1_s317c1
- Derived from:
- ccsbBroadEn_05117
- DNA Barcode:
- GCCTGGGCGCCTGACCTGTTGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RETREG3 (162427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465478
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 162427 | RETREG3 | reticulophagy regulator fam... | NM_178126.4 | 100% | 100% | |
| 2 | human | 162427 | RETREG3 | reticulophagy regulator fam... | XM_011524440.2 | 79.1% | 79.1% | 0_1ins291 |
| 3 | human | 162427 | RETREG3 | reticulophagy regulator fam... | NR_026697.2 | 35.8% | 1_103del;447_448ins31;1471_3778del | |
| 4 | mouse | 67998 | Retreg3 | reticulophagy regulator fam... | NM_026501.2 | 90.3% | 91.2% | (many diffs) |
| 5 | mouse | 67998 | Retreg3 | reticulophagy regulator fam... | NM_028933.3 | 54.7% | 55.3% | (many diffs) |
| 6 | mouse | 67998 | Retreg3 | reticulophagy regulator fam... | XM_006534032.3 | 53% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaggccgaa ggggttccca cgaccccagg cccggcttcg gggtcgactt 121 tcaggggccg ccgagatgtg tcaggctcct gggagcggga ccagcaggtt gaggcggcgc 181 agcgggccct ggtggaggtg ctggggcctt acgagcctct gctgagtcgg gtgcaggcag 241 ccctggtgtg ggagcggcca gctaggagcg ctctgtggtg cctggggctg aacgcggctt 301 tctggttttt tgccctgaca tctcttcgtc ttgtgttttt acttgcattt ggcttgatga 361 tcattgtgtg tattgatcaa tggaagaaca aaatctggcc tgaaataaaa gtgccaagac 421 ccgacgcatt agacaatgag agctggggct ttgtgcaccc tcggttgctc agcgtgcccg 481 agctctgcca ccatgtagct gaagtctggg ttagtgggac cattttcata aggaatgttt 541 tgcttttcaa aaagcaaaac ccaggcaagt tctgcttgct gagctgtggg atactgacct 601 ttttggctgt cttgggccgc tacgtccctg ggcttctgct gtcctacttg atgcttgtca 661 ctgtcatgat gtggcccctt gctgtgtacc accgactgtg ggatcgagca tatgtgcggc 721 tgaagccagc tctgcagcgg ctagacttca gtgtccgtgg ctacatgatg tccaagcaga 781 gagagagaca attacgccgc agagctctcc acccagaacg agccatggac aaccacagtg 841 acagcgaaga ggagcttgct gccttctgtc ctcagctgga cgattctact gttgccaggg 901 aattggccat cacagactct gagcactcag acgctgaagt ctcctgtaca gacaatggca 961 cattcaatct ttcaaggggc caaacacctc taacggaagg ctctgaagac ctagatggtc 1021 acagtgatcc agaggaatcc tttgccagag accttccaga cttcccttcc attaatatgg 1081 atcctgctgg cctggatgat gaggacgaca ctagcattgg catgcccagc ttgatgtacc 1141 gttcTCCGCC AGGGGCTGAG GAGCCCCAGG CCCCACCTGC CAGCCGGGAC GAGGCTGCGC 1201 TGCCGGAGCT CCTGCTTGGT GCTCTTCCTG TAGGATCCAA CCTCACCAGC AACCTTGCCA 1261 GCCTGGTCTC CCAGGGTATG ATTCAGCTGG CCTTGTCAGG GGCCTCCCAA CCAGGCCCTT 1321 CTGGAGCACC TGCCCAGAGA GCAACGAGAG GCTTCCTCCG GTCCCCCAGT TCAGACCTGG 1381 ACACTGATGC TGAGGGGGAT GACTTTGAAC TTCTGGACCA GTCGGAGCTG AGTCAGCTGG 1441 ACCCTGCCAG TTCTAGGAGC CACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CCTGGGCGCC 1621 TGACCTGTTG ATTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att