Construct: ORF TRCN0000465487
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014454.1_s317c1
- Derived from:
- ccsbBroadEn_04957
- DNA Barcode:
- AGTTACTTACTTAGACCATTCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SREK1 (140890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465487
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001323527.2 | 100% | 100% | |
| 2 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_139168.3 | 100% | 100% | |
| 3 | human | 140890 | SREK1 | splicing regulatory glutami... | XM_011543171.3 | 100% | 100% | |
| 4 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001270492.2 | 99.8% | 99.8% | 1230_1231insAGC |
| 5 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001077199.3 | 81.4% | 81.4% | 1_348del |
| 6 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001323529.2 | 81.2% | 81.2% | 1_348del;1578_1579insAGC |
| 7 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001323534.2 | 70.6% | 70.6% | 0_1ins447 |
| 8 | human | 140890 | SREK1 | splicing regulatory glutami... | XM_017009056.1 | 70.6% | 70.6% | 0_1ins447 |
| 9 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001323535.2 | 70.4% | 70.4% | 0_1ins447;783_784insAGC |
| 10 | human | 140890 | SREK1 | splicing regulatory glutami... | NM_001323533.2 | 67.6% | 66% | 1_348del;1580_1581ins145;1614_1615ins113 |
| 11 | human | 140890 | SREK1 | splicing regulatory glutami... | XM_017009055.2 | 62% | 62% | 1_348del;1120_1121ins363 |
| 12 | human | 140890 | SREK1 | splicing regulatory glutami... | XR_948236.3 | 56.4% | 1_422del;957_1131del;1794_1795ins327 | |
| 13 | mouse | 218543 | Srek1 | splicing regulatory glutami... | NM_172592.2 | 85% | 86.3% | (many diffs) |
| 14 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XM_006517607.3 | 85% | 86.3% | (many diffs) |
| 15 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XM_017315497.1 | 85% | 86.3% | (many diffs) |
| 16 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XM_006517606.2 | 69.3% | 70.3% | (many diffs) |
| 17 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XM_006517608.3 | 58.2% | 58.5% | (many diffs) |
| 18 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XM_017315498.1 | 58.2% | 58.5% | (many diffs) |
| 19 | mouse | 218543 | Srek1 | splicing regulatory glutami... | XR_001780780.1 | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1593
- ORF length:
- 1524
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaagtctg atgcctggtg caggattgct tccaataccg accccaaatc 121 ctttgactac tcttggtgtt tcacttagca gtttgggagc tataccagca gcagcactag 181 accccaacat tgcaacactt ggagagatac cacagccacc acttatggga aacgtggatc 241 cttccaaaat agatgaaatt aggagaacgg tttatgttgg aaatctgaat tcccagacaa 301 cgacagctga tcaactactt gaatttttta aacaagttgg agaagtgaag tttgtgcgga 361 tggcaggtga tgagactcag ccaactcggt ttgcttttgt ggaatttgca gaccaaaatt 421 ctgtaccaag ggcccttgct tttaatggag ttatgtttgg agacaggcca ctgaaaataa 481 atcactccaa caatgcaata gtaaaacccc ctgagatgac acctcaggct gcagctaagg 541 agttagaaga agtaatgaag cgagtacgag aagctcagtc atttatctca gcagctattg 601 aaccagagtc tggaaagagc aatgaaagaa aaggcggtcg atctcgttcc catactcgct 661 caaaatccag gtctagctca aaatcccatt ctagaaggaa aagatcacaa tcaaaacaca 721 ggagtagatc ccataataga tcacgttcaa gacagaaaga cagacgtaga tctaagagcc 781 cacataaaaa acgctctaaa tcaagggaga gacggaagtc aaggagtcgt tcgcattcac 841 gggacaagag aaaagacact cgagaaaaga tcaaggaaaa ggaaagagtg aaagagaaag 901 acagggaaaa ggagagagag agggaaaagg aacgtgaaaa agaaaaggaa cggggtaaaa 961 acaaagaccg ggacaaggaa cgggaaaagg accgggaaaa agacaaggaa aaggacagag 1021 agagagaacg ggaaaaagag catgagaagg atcgagacaa agagaaggaa aaggaacagg 1081 acaaagaaaa ggaacgagaa aaagacagat ccaaagagat agatgaaaaa agaaagaagg 1141 ataaaaaatc cagaacacca cccaggagtt acaatgcatc gcgaagatct cgtagttcca 1201 gcagggaaag gcgtaggagg aggagcagga gttcttccag atcgccaaga acatcaaaaa 1261 ccataaaaag gaaatcttct agatctccgt cccccaggag cagaaataag aaggataaaa 1321 agagagaaaa agaaagggac CACATCAGTG AAAGAAGAGA GAGAGAACGT TCAACGTCTA 1381 TGAGAAAGAG TTCTAATGAT AGAGATGGGA AGGAGAAGTT GGAGAAGAAC AGTACTTCAC 1441 TTAAAGAGAA AGAGCACAAT AAAGAACCAG ATTCAAGTGT GAGCAAAGAA GTAGATGACA 1501 AGGATGCACC AAGGACTGAG GAAAACAAAA TACAGCACAA TGGGAATTGT CAGCTGAATG 1561 AAGAAAACCT CTCTACCAAA ACAGAAGCAG TATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 1741 TTACTTACTT AGACCATTCT ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt