Construct: ORF TRCN0000465490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016302.1_s317c1
- Derived from:
- ccsbBroadEn_09048
- DNA Barcode:
- GAGATCAGAGCTTCTTTAACGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM177 (80775)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_001105198.1 | 99.7% | 99.3% | 94A>G;801C>G |
| 2 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_001105199.2 | 99.6% | 99.3% | 94A>G;267A>G;801C>G |
| 3 | human | 80775 | TMEM177 | transmembrane protein 177 | NM_030577.3 | 99.6% | 99.3% | 94A>G;267A>G;801C>G |
| 4 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959349.1 | 41.6% | (many diffs) | |
| 5 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959347.1 | 38% | (many diffs) | |
| 6 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959346.1 | 37.7% | (many diffs) | |
| 7 | human | 80775 | TMEM177 | transmembrane protein 177 | XR_002959348.1 | 33.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 999
- ORF length:
- 933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aggtcccctg tggcggaccg cagcatttgt gcagagacac aggacaggcc 121 tcttggtggg ttcctgtgca ggcctgtttg gagttccagt ctcgtaccac ctcttcccgg 181 atcccgtggt ccaatggctc taccagtact ggcctcaggg ccagccagct ccgctccctc 241 cacagctgca gagcctcttc caagaggtgc tacaggacat aggtgttcct tcaggccatt 301 gctacaagcc cttcaccacc ttcaccttcc agcctgtgag tgcaggcttc ccaagactcc 361 ctgctggggc tgtggtgggc atccctgcca gtttcttggg agacctagtg atcaacacta 421 accatcccgt ggtcatacat gggcatacag tggactggcg gagcccagca ggcgcccggc 481 tgagagcttc cctgaccttg tcccgtgaag cccagaagtt cgccttggcc agggaagtgg 541 tgtacctgga aagcagtacc actgccgtgc acgccctgct ggccccagct tgcctggcag 601 ggacctgggc actgggcgtg ggtgccaagt acaccctggg gctccatgca ggccccatga 661 atttacgggc tgccttcagc ttggtggcag cagtggcagg ctttgtggcc TACGCCTTCT 721 CCCAGGATTC TCTCACTCAT GCCGTGGAGT CCTGGCTGGA CCGCCGCACG GCCTCCCTCT 781 CTGCAGCCTA TGCCTGTGGT GGAGTGGAGT TCTATGAGAA GCTTCTGTCG GGCAACCTGG 841 CCCTGCGCAG TCTCTTGGGC AAAGAGGGGG AGAAGCTGTA TACACCCAGC GGGAACATCG 901 TCCCCAGACA CTTGTTCCGA ATCAAACATT TACCCTACAC CACCCGCCGG GACTCTGTGC 961 TGCAGATGTG GAGGGGGATG CTCAATCCGG GCCGCTCCTG CCCAACTTTC TTGTACAAAG 1021 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1081 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1141 ACGAGAGATC AGAGCTTCTT TAACGTAAAC GCGTTAAGTC gacaatcaac ctctggatta 1201 caaaatttgt gaaagatt