Construct: ORF TRCN0000465494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002666.1_s317c1
- Derived from:
- ccsbBroadEn_04452
- DNA Barcode:
- CAGACCACATTCCGAAGATTATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAEL (84944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84944 | MAEL | maelstrom spermatogenic tra... | NM_032858.3 | 100% | 100% | |
| 2 | human | 84944 | MAEL | maelstrom spermatogenic tra... | XM_011510067.1 | 99.3% | 99.3% | 313_314insAAGGTGATC |
| 3 | human | 84944 | MAEL | maelstrom spermatogenic tra... | XM_006711583.1 | 96.3% | 96.3% | 0_1ins46;2delT |
| 4 | human | 84944 | MAEL | maelstrom spermatogenic tra... | NM_001286377.1 | 92.8% | 92.8% | 131_132ins93 |
| 5 | human | 84944 | MAEL | maelstrom spermatogenic tra... | NM_001286378.1 | 87% | 87% | 0_1ins168 |
| 6 | human | 84944 | MAEL | maelstrom spermatogenic tra... | XM_017002602.1 | 87% | 87% | 0_1ins168 |
| 7 | human | 84944 | MAEL | maelstrom spermatogenic tra... | XM_017002603.1 | 87% | 87% | 0_1ins168 |
| 8 | human | 84944 | MAEL | maelstrom spermatogenic tra... | XM_011510068.1 | 86.4% | 86.4% | 0_1ins168;145_146insAAGGTGATC |
| 9 | mouse | 98558 | Mael | maelstrom spermatogenic tra... | NM_175296.4 | 89.6% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1368
- ORF length:
- 1302
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gaaccgtaag gccagccgga atgcttacta tttcttcgtg caggagaaga 121 tccccgaact acggcgacga ggcctgcctg tggctcgcgt tgctgatgcc atcccttact 181 gctcctcaga ctgggcgctt ctgagggagg aagaaaagga gaaatacgca gaaatggctc 241 gagaatggag ggccgctcag ggaaaggacc ctgggccctc agagaagcag aaacctgttt 301 tcacaccact gaggaggcca ggcatgcttg taccaaagca gaatgtttca cctccagata 361 tgtcagcttt gtctttaaaa ggtgatcaag ctctccttgg aggcattttt tattttttga 421 acatttttag ccatggcgag ctacctcctc attgtgaaca gcgcttcctc ccttgtgaaa 481 ttggctgtgt taagtattct ctccaagaag gtattatggc agatttccac agttttataa 541 atcctggtga aattccacga ggatttcgat ttcattgtca ggctgcaagt gattctagtc 601 acaagattcc tatttcaaat tttgaacgtg ggcataacca agcaactgtg ttacaaaacc 661 tttatagatt tattcatccc aacccaggga actggccacc tatctactgc aagtctgatg 721 atagaaccag agtcaactgg tgtttgaagc atatggcaaa ggcatcagaa atcaggcaag 781 atctacaact tctcactgta gaggaccttg tagtggggat ctaccaacaa aaatttctca 841 aggagccctc taagacttgg attcgaagcc tcctagatgt ggccatgtgg gattattcta 901 gcaacacaag gtgcaagtgg catgaagaaa atgatattct cttctgtgct ttagctgttT 961 GCAAGAAGAT TGCGTACTGC ATCAGTAATT CTCTGGCCAC TCTCTTTGGA ATCCAGCTCA 1021 CAGAGGCTCA TGTACCACTA CAAGATTATG AGGCCAGCAA TAGTGTGACA CCCAAAATGG 1081 TTGTATTGGA TGCAGGGCGT TACCAGAAGC TAAGGGTTGG GAGTTCAGGA TTCTCTCATT 1141 TCAACTCTTC TAATGAGGAA CAAAGATCAA ACACACCCAT TGGTGACTAC CCATCTAGGG 1201 CAAAAATTTC TGGCCAAAAC AGCAGCGTTC GGGGAAGAGG AATTACCCGC TTACTAGAGA 1261 GCATTTCCAA TTCTTCCAGC AATATCCACA AATTCTCCAA CTGTGACACT TCACTCTCAC 1321 CTTACATGTC CCAAAAAGAT GGATACAAAT CTTTCTCTTC CTTATCTTAC CCAACTTTCT 1381 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1441 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1501 GTGGAAAGGA CGACAGACCA CATTCCGAAG ATTATTAACG CGTTAAGTCg acaatcaacc 1561 tctggattac aaaatttgtg aaagatt