Construct: ORF TRCN0000465496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014779.1_s317c1
- Derived from:
- ccsbBroadEn_12294
- DNA Barcode:
- TTTCGGCCACACCAAGTTGAAAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BARX1 (56033)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56033 | BARX1 | BARX homeobox 1 | NM_021570.4 | 39.3% | 39.3% | 1_462del |
| 2 | mouse | 12022 | Barx1 | BarH-like homeobox 1 | NM_007526.4 | 35.1% | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 390
- ORF length:
- 300
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGCCTGGA GAAACGCTTC GAGAAGCAGA 121 AGTACCTTTC CACGCCGGAC AGAATAGATC TTGCTGAGTC CCTGGGCCTG AGCCAGTTGC 181 AGGTGAAGAC GTGGTACCAG AATCGGAGGA TGAAGTGGAA GAAAATAGTG CTGCAGGGCG 241 GCGGCCTGGA GTCTCCCACC AAGCCCAAGG GGCGGCCCAA GAAGAACTCA ATTCCAACGA 301 GCGAGCAGCT TACTGAGCAG GAGCGCGCCA AGGATGCAGA GAAACCGGCG GAGGTGCCGG 361 GCGAGCCCAG CGACAGGAGC CGCGAGGACT GCCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTCG 541 GCCACACCAA GTTGAAAGTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt