Construct: ORF TRCN0000465500
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013132.1_s317c1
- Derived from:
- ccsbBroadEn_08355
- DNA Barcode:
- CTAACCAATAGCTGTCCCTCGACA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PELO (53918)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465500
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 53918 | PELO | pelota mRNA surveillance an... | NM_015946.5 | 99.9% | 99.7% | 661C>A |
| 2 | mouse | 105083 | Pelo | pelota homolog (Drosophila) | NM_134058.3 | 90.3% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1221
- ORF length:
- 1155
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctcgtgagg aagaacatcg agaaggacaa tgcgggccag gtgaccctgg 121 tccccgagga gcctgaggac atgtggcaca cttacaacct cgtgcaggtg ggcgacagcc 181 tgcgcgcctc caccatccgc aaggtacaga cagagtcctc cacgggcagc gtgggcagca 241 accgggtccg cactaccctc actctctgcg tggaggccat cgacttcgac tctcaagcct 301 gccagctgcg ggttaagggg accaacatcc aagagaatga gtatgtcaag atgggggctt 361 accacaccat cgagctggag cccaaccgcc agttcaccct ggccaagaag cagtgggata 421 gtgtggtact ggagcgcatc gagcaggcct gtgacccagc ctggagcgct gatgtggcgg 481 ctgtggtcat gcaggaaggc ctcgcccata tctgcttagt cactcccagc atgaccctca 541 ctcgggccaa ggtggaggtg aacatcccta ggaaaaggaa aggcaattgc tctcagcatg 601 accgggcctt ggagcggttc tatgaacagg tggtccaggc tatccagcgc cacatacact 661 ttgatgttgt aaagtgcatc ctggtggcca gcccaggatt tgtgagggag cagttctgcg 721 actacatgtt tcaacaagca gtgaagaccg acaacaaact gctcctggaa aaccggtcca 781 aatttcttca ggtacatgcc tcctccggac acaagtactc cctgaaagag gccctttgtg 841 accctactgt ggctagccgc ctttcagaca ctaaagctgc tggggaagtc aaagccttgg 901 atgacttcta taaaatgtta cagcatgaac cggatcgagc tttctatgga ctcaagcagg 961 tggagaaggc caatgaagcc atggcaattg acacattgct catcagcgat gagctcttca 1021 ggcatcagga tgtagccaca cggagccggt atgtgaggct ggtggacagt gtgaaagaga 1081 atgcaggcac cgttaggata ttctctagtc ttcacgtttc tggggaacag ctcagccagt 1141 tgactggggt agctgccatt cTCCGCTTCC CTGTTCCCGA ACTTTCTGAC CAAGAGGGTG 1201 ATTCCAGTTC TGAAGAGGAT TACCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1261 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1321 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTAAC CAATAGCTGT 1381 CCCTCGACAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt