Construct: ORF TRCN0000465509
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009728.1_s317c1
- Derived from:
- ccsbBroadEn_02630
- DNA Barcode:
- CCTGTGTACTCGTGCACAGTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BVES (11149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465509
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11149 | BVES | blood vessel epicardial sub... | NM_001199563.2 | 100% | 100% | |
| 2 | human | 11149 | BVES | blood vessel epicardial sub... | NM_007073.4 | 100% | 100% | |
| 3 | human | 11149 | BVES | blood vessel epicardial sub... | NM_147147.3 | 100% | 100% | |
| 4 | human | 11149 | BVES | blood vessel epicardial sub... | XM_011535398.2 | 100% | 100% | |
| 5 | mouse | 23828 | Bves | blood vessel epicardial sub... | NM_024285.2 | 84.6% | 87.2% | (many diffs) |
| 6 | mouse | 23828 | Bves | blood vessel epicardial sub... | XM_006512736.3 | 84.6% | 87.2% | (many diffs) |
| 7 | mouse | 23828 | Bves | blood vessel epicardial sub... | XM_006512737.2 | 84.6% | 87.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1146
- ORF length:
- 1080
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttatacagag tccagcccat tgagagaatc aactgccata ggttttacac 121 ctgagttaga aagtatcata cctgtgcctt ccaataagac cacttgtgaa aactggagag 181 agatacatca tctggttttt catgtagcaa atatttgttt tgcagttggg ttggttattc 241 caactactct tcaccttcat atgatatttc ttaggggaat gttaactcta ggatgtaccc 301 tttatatcgt ctgggccact ctctaccgat gtgccttgga tataatgatc tggaactctg 361 tgttcttggg tgtcaacatt ttgcatctgt cgtatctttt atacaagaag agaccggtaa 421 agattgaaaa ggaactcagt ggcatgtacc ggcgattgtt tgaaccactc cgtgtgcctc 481 cagatttgtt cagaagacta actggacagt tttgcatgat ccaaaccttg aaaaagggcc 541 aaacttatgc tgcagaggat aaaacctcag ttgatgaccg tctgagtatt ctcttgaagg 601 gaaaaatgaa ggtctcctat cgaggacatt ttctgcataa catttacccc tgtgccttta 661 tagattctcc tgaatttaga tcaactcaga tgcacaaagg tgaaaaattc caggtcacca 721 ttattgcaga tgataactgc agatttttat gctggtcaag agaaagatta acatactttc 781 tggaatcaga acctttcttg tatgaaatct ttaggtatct tattggaaaa gacatcacaa 841 ataagctcta ctcattgaat gatcccacct taaatgataa aaaagccaaa aagctggaac 901 atcagctcag cctctgcaca cagatctcca tgttGGAAAT GAGGAACAGT ATAGCCAGCT 961 CCAGTGACAG TGACGACGGC TTGCACCAGT TTCTTCGGGG TACCTCCAGC ATGTCCTCTC 1021 TTCATGTGTC ATCCCCACAC CAGCGAGCCT CTGCCAAGAT GAAACCGATA GAAGAAGGAG 1081 CAGAAGATGA TGATGACGTT TTTGAACCGG CATCTCCAAA TACATTGAAA GTCCATCAGC 1141 TGCCTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1201 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1261 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTGTGTAC TCGTGCACAG TCAACACGCG 1321 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt