Construct: ORF TRCN0000465511
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003036.1_s317c1
- Derived from:
- ccsbBroadEn_05368
- DNA Barcode:
- GCAAACATGCCGTACGGTATCAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NHLRC4 (283948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465511
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283948 | NHLRC4 | NHL repeat containing 4 | NM_001301159.2 | 100% | 100% | |
| 2 | human | 283948 | NHLRC4 | NHL repeat containing 4 | NM_176677.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 438
- ORF length:
- 369
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgggtctg gagggcccct gctgggtggg cccagggccT GATGGGGGCC 121 TTGCTGTGAG TGAGGAGTTT GGGGATGTGA GGCTGTTTGG CAGTGCCCGC CAACCCCTGG 181 GCTCCCTGGG GGGCTGGACG GGGCACACTT TCGGCTGCCC AGCGGGCATC TGCTCCAACT 241 CAGAGGGCAA TGTTATTGTG GCAGACGAGC AGAGGCGCCA GGTGACCCTG TTTCCCCGGG 301 CTGGGCCACC CATCTGCCTG GTGTCAGAGG GGCTTGGGCA GCCCTTGGGA GTGGCCTGTG 361 CACCCCAGGG CCAGCTCCTG GTGGCTGATG CCAAGGACAA CTCCATCAAG GTGTACCAGG 421 GCCTCAAGGA GCTGGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCAAACA TGCCGTACGG 601 TATCAAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt