Construct: ORF TRCN0000465521

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004986.1_s317c1
Derived from:
ccsbBroadEn_04045
DNA Barcode:
TTTTACGTACGTCTACTGTTGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ALG12 (79087)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465521
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79087 ALG12 ALG12 alpha-1,6-mannosyltra... NM_024105.4 100% 100%
2 human 79087 ALG12 ALG12 alpha-1,6-mannosyltra... XM_017028936.1 88.2% 86.4% (many diffs)
3 human 79087 ALG12 ALG12 alpha-1,6-mannosyltra... XM_017028937.1 86.1% 81.3% (many diffs)
4 human 79087 ALG12 ALG12 alpha-1,6-mannosyltra... XM_011530371.2 72.1% 69.6% 992_993ins122;1056_1057ins286
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1530
ORF length:
1464
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tggaaagggg tcatcaggca ggcggcccct gctgctgggg ctgctggtgg
121ccgtagccac tgtccacctg gtcatctgtc cctacaccaa agtggaggag agcttcaacc
181tgcaggccac acatgacctg ctctaccact ggcaagacct ggagcagtac gaccatcttg
241agttccccgg agtcgtcccc aggacgttcc tcgggccagt ggtgatcgca gtgttctcca
301gccccgcggt ttacgtgctt tcgctgttag aaatgtccaa gttttactct cagctaatag
361ttagaggagt gcttggactc ggcgtgattt ttggactctg gacgttacaa aaggaagtga
421gacggcactt cggggccatg gtggccacca tgttctgctg ggtgacggcc atgcagttcc
481acctgatgtt ctactgcacg cggacactgc ccaatgtgct ggccctgcct gtagtcctgc
541tggccctcgc ggcctggctg cggcacgagt gggcccgctt catctggctg tcagccttcg
601ccatcatcgt gttcagggtg gagctgtgcc tgttcctggg cctcctgctg ctgctggcct
661tgggcaaccg aaaggtttct gtagtcagag cccttcgcca cgccgtcccg gcagggatcc
721tctgtttagg actgacggtt gctgtggact cttatttttg gcggcagctc acttggccgg
781aaggaaaggt gctttggtac aacactgtcc tgaacaaaag ctccaactgg gggacctccc
841cgctgctgtg gtacttctac tcagccctgc cccgcggcct gggctgcagc ctgctcttca
901tccccctggg cttggtagac agaaggacgc acgcgccgac ggtgctggca ctgggcttca
961tggcactcta ctccctcctg ccacacaagg agctacgctt catcatctat gccttcccca
1021tgctcaacat cacggctgcc agaggctgct cctacctgct gaataactat aaaaagtctt
1081ggctgtacaa agcggggtct ctgcttgtga tcggacacct cgtggtgaat gccgcctacT
1141CAGCCACGGC CCTGTATGTG TCCCATTTCA ACTACCCAGG TGGCGTCGCA ATGCAGAGGC
1201TGCACCAGCT GGTGCCCCCC CAGACAGACG TCCTTCTGCA CATTGACGTG GCAGCCGCCC
1261AGACAGGTGT GTCTCGGTTT CTCCAAGTCA ACAGCGCCTG GAGGTACGAC AAGAGGGAGG
1321ATGTGCAGCC GGGGACAGGC ATGCTGGCAT ACACACACAT CCTCATGGAG GCGGCCCCTG
1381GGCTCCTGGC CCTCTACAGG GACACACACC GGGTCCTGGC CAGCGTCGTG GGGACCACAG
1441GTGTGAGTCT GAACCTGACC CAACTGCCCC CCTTCAACGT CCACCTGCAG ACAAAGCTGG
1501TGCTTCTGGA GAGGCTCCCC CGGCCGTCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1561TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1621CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTTA
1681CGTACGTCTA CTGTTGTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1741tgaaagatt

Download FASTA (ORF) (Full)