Construct: ORF TRCN0000465521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004986.1_s317c1
- Derived from:
- ccsbBroadEn_04045
- DNA Barcode:
- TTTTACGTACGTCTACTGTTGTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ALG12 (79087)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79087 | ALG12 | ALG12 alpha-1,6-mannosyltra... | NM_024105.4 | 100% | 100% | |
| 2 | human | 79087 | ALG12 | ALG12 alpha-1,6-mannosyltra... | XM_017028936.1 | 88.2% | 86.4% | (many diffs) |
| 3 | human | 79087 | ALG12 | ALG12 alpha-1,6-mannosyltra... | XM_017028937.1 | 86.1% | 81.3% | (many diffs) |
| 4 | human | 79087 | ALG12 | ALG12 alpha-1,6-mannosyltra... | XM_011530371.2 | 72.1% | 69.6% | 992_993ins122;1056_1057ins286 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1530
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tggaaagggg tcatcaggca ggcggcccct gctgctgggg ctgctggtgg 121 ccgtagccac tgtccacctg gtcatctgtc cctacaccaa agtggaggag agcttcaacc 181 tgcaggccac acatgacctg ctctaccact ggcaagacct ggagcagtac gaccatcttg 241 agttccccgg agtcgtcccc aggacgttcc tcgggccagt ggtgatcgca gtgttctcca 301 gccccgcggt ttacgtgctt tcgctgttag aaatgtccaa gttttactct cagctaatag 361 ttagaggagt gcttggactc ggcgtgattt ttggactctg gacgttacaa aaggaagtga 421 gacggcactt cggggccatg gtggccacca tgttctgctg ggtgacggcc atgcagttcc 481 acctgatgtt ctactgcacg cggacactgc ccaatgtgct ggccctgcct gtagtcctgc 541 tggccctcgc ggcctggctg cggcacgagt gggcccgctt catctggctg tcagccttcg 601 ccatcatcgt gttcagggtg gagctgtgcc tgttcctggg cctcctgctg ctgctggcct 661 tgggcaaccg aaaggtttct gtagtcagag cccttcgcca cgccgtcccg gcagggatcc 721 tctgtttagg actgacggtt gctgtggact cttatttttg gcggcagctc acttggccgg 781 aaggaaaggt gctttggtac aacactgtcc tgaacaaaag ctccaactgg gggacctccc 841 cgctgctgtg gtacttctac tcagccctgc cccgcggcct gggctgcagc ctgctcttca 901 tccccctggg cttggtagac agaaggacgc acgcgccgac ggtgctggca ctgggcttca 961 tggcactcta ctccctcctg ccacacaagg agctacgctt catcatctat gccttcccca 1021 tgctcaacat cacggctgcc agaggctgct cctacctgct gaataactat aaaaagtctt 1081 ggctgtacaa agcggggtct ctgcttgtga tcggacacct cgtggtgaat gccgcctacT 1141 CAGCCACGGC CCTGTATGTG TCCCATTTCA ACTACCCAGG TGGCGTCGCA ATGCAGAGGC 1201 TGCACCAGCT GGTGCCCCCC CAGACAGACG TCCTTCTGCA CATTGACGTG GCAGCCGCCC 1261 AGACAGGTGT GTCTCGGTTT CTCCAAGTCA ACAGCGCCTG GAGGTACGAC AAGAGGGAGG 1321 ATGTGCAGCC GGGGACAGGC ATGCTGGCAT ACACACACAT CCTCATGGAG GCGGCCCCTG 1381 GGCTCCTGGC CCTCTACAGG GACACACACC GGGTCCTGGC CAGCGTCGTG GGGACCACAG 1441 GTGTGAGTCT GAACCTGACC CAACTGCCCC CCTTCAACGT CCACCTGCAG ACAAAGCTGG 1501 TGCTTCTGGA GAGGCTCCCC CGGCCGTCCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1561 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1621 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTTA 1681 CGTACGTCTA CTGTTGTTTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1741 tgaaagatt