Construct: ORF TRCN0000465524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008663.1_s317c1
- Derived from:
- ccsbBroadEn_15862
- DNA Barcode:
- GCCATCGTCATACTCGTTGGTTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC10 (54431)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_018981.4 | 99.9% | 99.8% | 1938T>G;2241A>G |
| 2 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NM_001271581.3 | 94.1% | 94% | 846_847ins138;1800T>G;2103A>G |
| 3 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073365.2 | 11.3% | (many diffs) | |
| 4 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073366.2 | 11.3% | (many diffs) | |
| 5 | human | 54431 | DNAJC10 | DnaJ heat shock protein fam... | NR_073367.2 | 10.8% | (many diffs) | |
| 6 | mouse | 66861 | Dnajc10 | DnaJ heat shock protein fam... | NM_024181.2 | 87.3% | 90.4% | (many diffs) |
| 7 | mouse | 66861 | Dnajc10 | DnaJ heat shock protein fam... | XR_001783164.1 | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2448
- ORF length:
- 2379
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagtctgg ttaaataaag atgactatat cagagacttg aaaaggatca 121 ttctctgttt tctgatagtg tatatggcca ttttagtggg cacagatcag gatttttaca 181 gtttacttgg agtgtccaaa actgcaagca gtagagaaat aagacaagct ttcaagaaat 241 tggcattgaa gttacatcct gataaaaacc cgaataaccc aaatgcacat ggcgattttt 301 taaaaataaa tagagcatat gaagtactca aagatgaaga tctacggaaa aagtatgaca 361 aatatggaga aaagggactt gaggataatc aaggtggcca gtatgaaagc tggaactatt 421 atcgttatga ttttggtatt tatgatgatg atcctgaaat cataacattg gaaagaagag 481 aatttgatgc tgctgttaat tctggagaac tgtggtttgt aaatttttac tccccaggct 541 gttcacactg ccatgattta gctcccacat ggagagactt tgctaaagaa gtggatgggt 601 tacttcgaat tggagctgtt aactgtggtg atgatagaat gctttgccga atgaaaggag 661 tcaacagcta tcccagcctc ttcatttttc ggtctggaat ggccccagtg aaatatcatg 721 gagacagatc aaaggagagt ttagtgagtt ttgcaatgca gcatgttaga agtacagtga 781 cagaactttg gacaggaaat tttgtcaact ccatacaaac tgcttttgct gctggtattg 841 gctggctgat cactttttgt tcaaaaggag gagattgttt gacttcacag acacgactca 901 ggcttagtgg catgttggat ggtcttgtta atgtaggatg gatggactgt gccacccagg 961 ataacctttg taaaagctta gatattacaa caagtactac tgcttatttt cctcctggag 1021 ccactttaaa taacaaagag aaaaacagta ttttgtttct caactcattg gatgctaaag 1081 aaatatattt ggaagtaata cataatcttc cagattttga actactttcg gcaaacacac 1141 tagaggatcg tttggctcat catcggtggc tgttattttt tcattttgga aaaaatgaaa 1201 attcaaatga tcctgagctg aaaaaactaa aaactctact taaaaatgat catattcaag 1261 ttggcaggtt tgactgttcc tctgcaccag acatctgtag taatctgtat gtttttcagc 1321 cgtctctagc agtatttaaa ggacaaggaa ccaaagaata tgaaattcat catggaaaga 1381 agattctata tgatatactt gcctttgcca aagaaagtgt gaattctcat gttaccacgc 1441 ttggacctca aaattttcct gccaatgaca aagaaccatg gcttgttgat ttctttgccc 1501 cctggtgtcc accatgtcga gctttactac cagagttacg aagagcatca aatcttcttt 1561 atggtcagct taagtttggt acactagatt gtacagttca tgagggactc tgtaacatgt 1621 ataacattca ggcttatcca acaacagtgg tattcaacca gtccaacatt catgagtatg 1681 aaggacatca ctctgctgaa caaatcttgg agttcataga ggatcttatg aatccttcag 1741 tggtctccct tacacccacc accttcaacg aactagttac acaaagaaaa cacaacgaag 1801 tctggatggt tgatttctat tctccgtggt gtcatccttg ccaagtctta atgccagaat 1861 ggaaaagaat ggcccggaca ttaactggac tgatcaacgt gggcagtata gattgccaac 1921 agtatcattc tttttgtgcc caggaaaacg ttcaaagata ccctgagata agattttttc 1981 ccccaaaatc aaataaagct tatcagtatc acagttacaa tggttggaat agggatgctt 2041 attccctgag aatctggggt ctaggatttt tacctcaagt atccacagat ctaacaccTC 2101 AGACTTTCAG TGAAAAAGTT CTACAAGGGA AAAATCATTG GGTGATTGAT TTCTATGCTC 2161 CTTGGTGTGG ACCTTGCCAG AATTTTGCTC CAGAATTTGA GCTCTTGGCT AGGATGATTA 2221 AAGGAAAAGT GAAAGCTGGA AAAGTAGACT GTCAGGCTTA TGCTCAGACA TGCCAGAAAG 2281 CTGGGATCAG GGCCTATCCA ACTGTTAAGT TTTATTTCTA CGAAAGAGCA AAGAGAAATT 2341 TTCAAGAAGA GCAGATAAAT ACCAGAGATG CAAAAGCAAT CGCTGCCTTA ATAAGTGAAA 2401 AATTGGAAAC TCTCCGAAAT CAAGGCAAGA GGAATAAGGA TGAACTTTTG CCAACTTTCT 2461 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 2521 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 2581 GTGGAAAGGA CGAGCCATCG TCATACTCGT TGGTTCCACG CGTTAAGTCg acaatcaacc 2641 tctggattac aaaatttgtg aaagatt