Construct: ORF TRCN0000465537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013196.1_s317c1
- Derived from:
- ccsbBroadEn_15423
- DNA Barcode:
- TCAACCGGGCACGCAACGACACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT1 (2589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2589 | GALNT1 | polypeptide N-acetylgalacto... | NM_020474.3 | 18.7% | 18.7% | 316_1677del |
| 2 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_001160404.1 | 17.7% | 18% | (many diffs) |
| 3 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | NM_013814.3 | 17.7% | 18% | (many diffs) |
| 4 | mouse | 14423 | Galnt1 | UDP-N-acetyl-alpha-D-galact... | XM_006525650.2 | 17.7% | 18% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aaaatttgca tactgcaagg tggtcctagc cacctccttg atttgggtac 121 tcttgGATAT GTTCCTGCTG CTTTACTTCA GTGAATGCAA CAAATGTGAT GAAAAAAAGG 181 AGAGAGGACT TCCTGCTGGA GATGTTCTAG AGCCAGTACA AAAGCCTCAT GAAGGTCCTG 241 GAGAAATGGG GAAACCAGTC GTCATTCCTA AAGAGGATCA AGAAAAGATG AAAGAGATGT 301 TTAAAATCAA TCAGTTCAAT TTAATGGCAA GTGAGATGAT TGCACTCAAC AGATCTTTAC 361 CAGATGTTAG GTTAGAAGGG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAA CCGGGCACGC 541 AACGACACAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt