Construct: ORF TRCN0000465553
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009003.1_s317c1
- Derived from:
- BRDN0000395145
- DNA Barcode:
- CTTCCTCATGCCCGGTCTTACAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FANCI (55215)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465553
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55215 | FANCI | FA complementation group I | NM_018193.3 | 99.9% | 99.7% | 23T>C;26C>G;3356A>T |
| 2 | human | 55215 | FANCI | FA complementation group I | XM_011521764.2 | 99.9% | 99.7% | 23T>C;26C>G;3356A>T |
| 3 | human | 55215 | FANCI | FA complementation group I | NM_001113378.1 | 95.4% | 95.2% | (many diffs) |
| 4 | human | 55215 | FANCI | FA complementation group I | XM_011521756.2 | 95.4% | 95.2% | (many diffs) |
| 5 | human | 55215 | FANCI | FA complementation group I | XM_011521757.2 | 95.4% | 95.2% | (many diffs) |
| 6 | human | 55215 | FANCI | FA complementation group I | XM_011521767.2 | 88.4% | 88.4% | 0_1ins279;2177_2356del;3257A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3873
- ORF length:
- 3804
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaccagaag attttatctc caggagcaga aaaaacagca gacaaactgc 121 aagaatttct tcaaaccctg agagaaggtg atttgactaa tctccttcag aatcaagcag 181 tgaaaggaaa agttgctgga gcactcctga gagccatctt caaaggttcc ccctgctctg 241 aggaagctgg aacacttagg agacgtaaga tatacacttg ttgtatccag ttggtggaat 301 cgggggattt gcagaaagaa atagcgtctg agatcatagg attactgatg ctggaggctc 361 accattttcc aggaccatta ttggttgaat tagccaatga gtttattagt gctgtcagag 421 aaggcagcct agtgaatgga aaatctttgg agttactacc tatcattctc actgccctgg 481 ctacgaaaaa ggaaaatctg gcttatggaa aaggtgtact gagtggggaa gaatgtaaga 541 aacagttgat taacaccctg tgttctggca ggtgggatca gcaatatgta atccaactca 601 cctccatgtt caaggatgtc cctctgactg cagaagaggt ggaatttgtg gtggaaaaag 661 cattgagcat gttctccaag atgaatcttc aagaaatacc acctttggtc tatcagcttc 721 tggttctctc ctccaaggga agcagaaaga gtgttttgga aggaatcata gccttcttca 781 gtgcactaga taagcagcac aatgaggaac agagtggtga cgagctattg gatgttgtca 841 ctgtgccatc aggtgaactt cgtcatgtgg aaggcaccat tattctacac attgtgtttg 901 ccatcaaatt ggactatgaa ctaggcagag aactcgtgaa acacttaaag gtaggacagc 961 aaggagattc caataataac ttaagtccct tcagcattgc tcttcttctg tctgtaacaa 1021 gaatacaaag atttcaggac caggtgcttg atcttttaaa gacttcggtt gtaaagagct 1081 ttaaggatct tcaactcctc caaggctcaa aatttcttca gaatctagtt cctcatagat 1141 cttatgtttc aaccatgatc ttggaagtag tgaagaatag cgttcatagc tgggaccatg 1201 ttactcaggg cctcgtagaa cttggtttca ttttgatgga ttcatatggg ccaaagaagg 1261 ttcttgatgg aaaaactatt gaaaccagcc caagtctttc tagaatgcca aaccagcatg 1321 catgtaagct cggagctaat atcctgttgg aaacttttaa gatccatgag atgatcagac 1381 aagaaatttt ggagcaggtc ctcaacaggg ttgttaccag agcatcttct cccatcagtc 1441 atttcttaga cctgctttca aatatcgtca tgtatgcacc cttagttctt caaagttgtt 1501 cttctaaagt cacagaagct tttgactatt tgtcctttct gccccttcag actgtacaaa 1561 ggctgcttaa ggcagtgcag ccccttctca aagtcagcat gtcaatgaga gactgcttga 1621 tacttgtcct tcggaaagct atgtttgcca accagcttga tgcccgaaaa tctgcagttg 1681 ctgggttttt gctgctcctg aagaacttta aagttttagg cagcctgtca tcctctcagt 1741 gcagtcagtc tctcagtgtc agtcaggttc atgtggatgt tcacagccat tacaattctg 1801 tcgccaatga aactttttgc cttgagatca tggatagttt gaggagatgc ttaagccagc 1861 aagctgatgt tcgactcatg ctttatgagg ggttttatga tgttcttcga aggaactctc 1921 agctggctaa ttcagtcatg caaactctgc tctcacagtt aaaacagttc tatgagccaa 1981 aacctgatct gctgcctcct ctgaaattag aagcttgtat tctgacccaa ggagataaga 2041 tctctctaca agaaccactg gattatctgc tgtgttgtat tcagcattgt ttggcctggt 2101 ataagaatac agtcataccc ttacagcagg gagaggagga agaggaggag gaagaggcat 2161 tctacgaaga cctagatgat atattggagt ccattactaa tagaatgatt aagagtgagc 2221 tggaagactt tgaactggat aaatcagcag atttttctca gagcaccagt attggcataa 2281 aaaataatat ctgtgctttt cttgtgatgg gagtttgtga ggttttaata gaatacaatt 2341 tctccataag tagtttcagt aagaataggt ttgaggacat tctgagctta tttatgtgtt 2401 acaaaaaact ctctgacatt cttaatgaaa aagcgggtaa agccaaaact aaaatggcca 2461 acaagacaag tgatagtctt ttgtccatga aatttgtgtc cagtcttctc actgctcttt 2521 tcagagtctt gctatggaga tacacttcaa ttcctacttc agtggaagag tcgggaaaga 2581 aagagaaagg aaagagcatc tcactgctgt gcttggaggg tttacagaaa atattcagtg 2641 ctgtgcaaca gttctatcag cccaagattc agcagtttct cagagctctg gatgtcacag 2701 ataaggaagg agaagagaga gaagatgcag atgtcagtgt cactcagaga acagcattcc 2761 agatccggca atttcagagg tccttgttga atttacttag cagtcaagag gaagatttta 2821 atagcaaaga agccctcctg ctagtcacgg ttcttaccag tttgtccaag ttactggagc 2881 cctcctctcc tcagtttgtg cagatgttat cctggacatc aaagatttgc aaggaaaaca 2941 gccgggagga tgccttgttt tgcaagagct tgatgaactt gctcttcagc ctgcatgttt 3001 cgtataagag tcctgtcatt ctgctgcgtg acttgtccca ggatatccac gggcatctgg 3061 gagatataga ccaggatgta gaggtggaga aaacaaacca ctttgcaata gtgaatttga 3121 gaacggctgc ccccactgtc tgtttacttg ttctgagtca ggccgagaag gttctagaag 3181 aagtggactg gctaatcacc aagcttaagg gacaagtgag ccaagaaacc ttatcagaag 3241 aggcctcttc tcaggcaacc ctaccaaatc agcctgttga gaaagctatc atcatgcaac 3301 tgggaactct gcttacattt ttccacgagc tggtgcagac agctctgcca tcaggcagct 3361 gtgtggacac cttgttaaag gacttgtgca aaatgtacac cacacttaca gcccttgtca 3421 gattttatct ccaggtgtgt cagagctccg gaggaattcc aaaaaatatg gaaaagctgg 3481 tgaagctgtc tggttctcat ctgacccccc tgtgttattc tttcatttct tacgtacaga 3541 ataagagtaa gagcctgaac tatacgggag agaaaaagga gaaacctgct gccgttgcca 3601 cagccatggc cagagttctt cgggaaacca agccaatccc taaccTCATC TTTGCCATAG 3661 AACAGTATGA AAAATTTCTC ATCCACCTTT CTAAGAAGTC CAAGGTGAAC CTGATGCAGC 3721 ACATGAAGCT CAGCACCTCA CGAGACTTCA AGATCAAAGG AAACATCCTA GACATGGTTC 3781 TTCGAGAGGA TGGTGAAGAT GAAAATGAAG AGGGCACTGC ATCAGAGCAT GGGGGACAGA 3841 ACAAAGAACC AGCCAAGAAG AAAAGGAAAA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 3901 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3961 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 4021 TCCTCATGCC CGGTCTTACA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 4081 ttgtgaaaga tt