Construct: ORF TRCN0000465553

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009003.1_s317c1
Derived from:
BRDN0000395145
DNA Barcode:
CTTCCTCATGCCCGGTCTTACAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FANCI (55215)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465553
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55215 FANCI FA complementation group I NM_018193.3 99.9% 99.7% 23T>C;26C>G;3356A>T
2 human 55215 FANCI FA complementation group I XM_011521764.2 99.9% 99.7% 23T>C;26C>G;3356A>T
3 human 55215 FANCI FA complementation group I NM_001113378.1 95.4% 95.2% (many diffs)
4 human 55215 FANCI FA complementation group I XM_011521756.2 95.4% 95.2% (many diffs)
5 human 55215 FANCI FA complementation group I XM_011521757.2 95.4% 95.2% (many diffs)
6 human 55215 FANCI FA complementation group I XM_011521767.2 88.4% 88.4% 0_1ins279;2177_2356del;3257A>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3873
ORF length:
3804
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggaccagaag attttatctc caggagcaga aaaaacagca gacaaactgc
121aagaatttct tcaaaccctg agagaaggtg atttgactaa tctccttcag aatcaagcag
181tgaaaggaaa agttgctgga gcactcctga gagccatctt caaaggttcc ccctgctctg
241aggaagctgg aacacttagg agacgtaaga tatacacttg ttgtatccag ttggtggaat
301cgggggattt gcagaaagaa atagcgtctg agatcatagg attactgatg ctggaggctc
361accattttcc aggaccatta ttggttgaat tagccaatga gtttattagt gctgtcagag
421aaggcagcct agtgaatgga aaatctttgg agttactacc tatcattctc actgccctgg
481ctacgaaaaa ggaaaatctg gcttatggaa aaggtgtact gagtggggaa gaatgtaaga
541aacagttgat taacaccctg tgttctggca ggtgggatca gcaatatgta atccaactca
601cctccatgtt caaggatgtc cctctgactg cagaagaggt ggaatttgtg gtggaaaaag
661cattgagcat gttctccaag atgaatcttc aagaaatacc acctttggtc tatcagcttc
721tggttctctc ctccaaggga agcagaaaga gtgttttgga aggaatcata gccttcttca
781gtgcactaga taagcagcac aatgaggaac agagtggtga cgagctattg gatgttgtca
841ctgtgccatc aggtgaactt cgtcatgtgg aaggcaccat tattctacac attgtgtttg
901ccatcaaatt ggactatgaa ctaggcagag aactcgtgaa acacttaaag gtaggacagc
961aaggagattc caataataac ttaagtccct tcagcattgc tcttcttctg tctgtaacaa
1021gaatacaaag atttcaggac caggtgcttg atcttttaaa gacttcggtt gtaaagagct
1081ttaaggatct tcaactcctc caaggctcaa aatttcttca gaatctagtt cctcatagat
1141cttatgtttc aaccatgatc ttggaagtag tgaagaatag cgttcatagc tgggaccatg
1201ttactcaggg cctcgtagaa cttggtttca ttttgatgga ttcatatggg ccaaagaagg
1261ttcttgatgg aaaaactatt gaaaccagcc caagtctttc tagaatgcca aaccagcatg
1321catgtaagct cggagctaat atcctgttgg aaacttttaa gatccatgag atgatcagac
1381aagaaatttt ggagcaggtc ctcaacaggg ttgttaccag agcatcttct cccatcagtc
1441atttcttaga cctgctttca aatatcgtca tgtatgcacc cttagttctt caaagttgtt
1501cttctaaagt cacagaagct tttgactatt tgtcctttct gccccttcag actgtacaaa
1561ggctgcttaa ggcagtgcag ccccttctca aagtcagcat gtcaatgaga gactgcttga
1621tacttgtcct tcggaaagct atgtttgcca accagcttga tgcccgaaaa tctgcagttg
1681ctgggttttt gctgctcctg aagaacttta aagttttagg cagcctgtca tcctctcagt
1741gcagtcagtc tctcagtgtc agtcaggttc atgtggatgt tcacagccat tacaattctg
1801tcgccaatga aactttttgc cttgagatca tggatagttt gaggagatgc ttaagccagc
1861aagctgatgt tcgactcatg ctttatgagg ggttttatga tgttcttcga aggaactctc
1921agctggctaa ttcagtcatg caaactctgc tctcacagtt aaaacagttc tatgagccaa
1981aacctgatct gctgcctcct ctgaaattag aagcttgtat tctgacccaa ggagataaga
2041tctctctaca agaaccactg gattatctgc tgtgttgtat tcagcattgt ttggcctggt
2101ataagaatac agtcataccc ttacagcagg gagaggagga agaggaggag gaagaggcat
2161tctacgaaga cctagatgat atattggagt ccattactaa tagaatgatt aagagtgagc
2221tggaagactt tgaactggat aaatcagcag atttttctca gagcaccagt attggcataa
2281aaaataatat ctgtgctttt cttgtgatgg gagtttgtga ggttttaata gaatacaatt
2341tctccataag tagtttcagt aagaataggt ttgaggacat tctgagctta tttatgtgtt
2401acaaaaaact ctctgacatt cttaatgaaa aagcgggtaa agccaaaact aaaatggcca
2461acaagacaag tgatagtctt ttgtccatga aatttgtgtc cagtcttctc actgctcttt
2521tcagagtctt gctatggaga tacacttcaa ttcctacttc agtggaagag tcgggaaaga
2581aagagaaagg aaagagcatc tcactgctgt gcttggaggg tttacagaaa atattcagtg
2641ctgtgcaaca gttctatcag cccaagattc agcagtttct cagagctctg gatgtcacag
2701ataaggaagg agaagagaga gaagatgcag atgtcagtgt cactcagaga acagcattcc
2761agatccggca atttcagagg tccttgttga atttacttag cagtcaagag gaagatttta
2821atagcaaaga agccctcctg ctagtcacgg ttcttaccag tttgtccaag ttactggagc
2881cctcctctcc tcagtttgtg cagatgttat cctggacatc aaagatttgc aaggaaaaca
2941gccgggagga tgccttgttt tgcaagagct tgatgaactt gctcttcagc ctgcatgttt
3001cgtataagag tcctgtcatt ctgctgcgtg acttgtccca ggatatccac gggcatctgg
3061gagatataga ccaggatgta gaggtggaga aaacaaacca ctttgcaata gtgaatttga
3121gaacggctgc ccccactgtc tgtttacttg ttctgagtca ggccgagaag gttctagaag
3181aagtggactg gctaatcacc aagcttaagg gacaagtgag ccaagaaacc ttatcagaag
3241aggcctcttc tcaggcaacc ctaccaaatc agcctgttga gaaagctatc atcatgcaac
3301tgggaactct gcttacattt ttccacgagc tggtgcagac agctctgcca tcaggcagct
3361gtgtggacac cttgttaaag gacttgtgca aaatgtacac cacacttaca gcccttgtca
3421gattttatct ccaggtgtgt cagagctccg gaggaattcc aaaaaatatg gaaaagctgg
3481tgaagctgtc tggttctcat ctgacccccc tgtgttattc tttcatttct tacgtacaga
3541ataagagtaa gagcctgaac tatacgggag agaaaaagga gaaacctgct gccgttgcca
3601cagccatggc cagagttctt cgggaaacca agccaatccc taaccTCATC TTTGCCATAG
3661AACAGTATGA AAAATTTCTC ATCCACCTTT CTAAGAAGTC CAAGGTGAAC CTGATGCAGC
3721ACATGAAGCT CAGCACCTCA CGAGACTTCA AGATCAAAGG AAACATCCTA GACATGGTTC
3781TTCGAGAGGA TGGTGAAGAT GAAAATGAAG AGGGCACTGC ATCAGAGCAT GGGGGACAGA
3841ACAAAGAACC AGCCAAGAAG AAAAGGAAAA AATTGCCAAC TTTCTTGTAC AAAGTGGTTG
3901ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA
3961GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT
4021TCCTCATGCC CGGTCTTACA ATACGCGTTA AGTCgacaat caacctctgg attacaaaat
4081ttgtgaaaga tt

Download FASTA (ORF) (Full)