Construct: ORF TRCN0000465572
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011368.1_s317c1
- Derived from:
- ccsbBroadEn_07175
- DNA Barcode:
- GATCAGCCTGATCAAGTAATAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFAND5 (7763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465572
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_001278244.1 | 100% | 100% | |
| 2 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_001102420.3 | 99.8% | 100% | 153A>G |
| 3 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_001102421.3 | 99.8% | 100% | 153A>G |
| 4 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_001278243.2 | 99.8% | 100% | 153A>G |
| 5 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_001278245.2 | 99.8% | 100% | 153A>G |
| 6 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | NM_006007.4 | 99.8% | 100% | 153A>G |
| 7 | human | 7763 | ZFAND5 | zinc finger AN1-type contai... | XM_024447665.1 | 99.8% | 100% | 153A>G |
| 8 | mouse | 22682 | Zfand5 | zinc finger, AN1-type domain 5 | NM_009551.5 | 96.7% | 98.5% | (many diffs) |
| 9 | mouse | 22682 | Zfand5 | zinc finger, AN1-type domain 5 | XM_006527058.3 | 96.7% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttcgcatggc tcaggagact aaccagaccc cggggcccat gctgtgtagc acaggatgtg 121 gcttttatgg aaatcctagg acaaatggaa tgtgttcagt ttgctacaaa gaacatcttc 181 agaggcagca aaatagtggc agaatgagcc caatggggac agctagtggt tccaacagtc 241 ctacctcaga ttctgcatct gtacagagag cagacactag cttaaacaac tgtgaaggtg 301 ctgctggcag cacatcTGAA AAATCAAGAA ATGTGCCTGT GGCTGCCTTG CCTGTAACTC 361 AGCAAATGAC AGAAATGAGC ATTTCAAGAG AGGACAAAAT AACTACCCCG AAAACAGAGG 421 TGTCAGAGCC AGTTGTCACT CAGCCCAGTC CATCAGTTTC TCAGCCCAGT ACTTCTCAGA 481 GTGAAGAAAA AGCTCCTGAA TTGCCCAAAC CAAAGAAAAA CAGATGTTTC ATGTGCAGAA 541 AGAAAGTTGG TCTTACAGGG TTTGACTGCC GATGTGGAAA TTTGTTTTGT GGACTTCACC 601 GTTACTCTGA CAAGCACAAC TGTCCGTATG ATTACAAAGC AGAAGCTGCA GCAAAAATCA 661 GAAAAGAGAA TCCAGTTGTT GTGGCTGAAA AAATTCAGAG AATATACGCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA GATCAGCCTG ATCAAGTAAT AAATACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt