Construct: ORF TRCN0000465593

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004789.1_s317c1
Derived from:
ccsbBroadEn_06580
DNA Barcode:
AGTATCACGGCTATGGGTACTGAC
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MGAT1 (4245)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465593
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114617.1 99.9% 99.7% 1304T>C
2 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114618.1 99.9% 99.7% 1304T>C
3 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114619.1 99.9% 99.7% 1304T>C
4 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001114620.1 99.9% 99.7% 1304T>C
5 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364377.2 99.9% 99.7% 1304T>C
6 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364379.2 99.9% 99.7% 1304T>C
7 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364380.2 99.9% 99.7% 1304T>C
8 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364381.2 99.9% 99.7% 1304T>C
9 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364382.2 99.9% 99.7% 1304T>C
10 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364383.2 99.9% 99.7% 1304T>C
11 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364384.2 99.9% 99.7% 1304T>C
12 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364385.2 99.9% 99.7% 1304T>C
13 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364386.2 99.9% 99.7% 1304T>C
14 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364387.2 99.9% 99.7% 1304T>C
15 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364388.2 99.9% 99.7% 1304T>C
16 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364389.2 99.9% 99.7% 1304T>C
17 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364390.2 99.9% 99.7% 1304T>C
18 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364391.2 99.9% 99.7% 1304T>C
19 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364392.2 99.9% 99.7% 1304T>C
20 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364393.2 99.9% 99.7% 1304T>C
21 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364394.2 99.9% 99.7% 1304T>C
22 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_001364395.2 99.9% 99.7% 1304T>C
23 human 4245 MGAT1 alpha-1,3-mannosyl-glycopro... NM_002406.4 99.9% 99.7% 1304T>C
24 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110148.1 84.3% 89.9% (many diffs)
25 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110149.1 84.3% 89.9% (many diffs)
26 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_001110150.1 84.3% 89.9% (many diffs)
27 mouse 17308 Mgat1 mannoside acetylglucosaminy... NM_010794.3 84.3% 89.9% (many diffs)
28 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532397.3 84.3% 89.9% (many diffs)
29 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532398.3 84.3% 89.9% (many diffs)
30 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532399.2 84.3% 89.9% (many diffs)
31 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_006532400.3 84.3% 89.9% (many diffs)
32 mouse 17308 Mgat1 mannoside acetylglucosaminy... XM_017314314.1 84.3% 89.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1401
ORF length:
1335
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgct gaagaagcag tctgcagggc ttgtgctgtg gggcgctatc ctctttgtgg
121cctggaatgc cctgctgctc ctcttcttct ggacgcgccc agcacctggc aggccaccct
181cagtcagcgc tctcgatggc gaccccgcca gcctcacccg ggaagtgatt cgcctggccc
241aagacgccga ggtggagctg gagcggcagc gtgggctgct gcagcagatc ggggatgccc
301tgtcgagcca gcgggggagg gtgcccaccg cggcccctcc cgcccagccg cgtgtgcctg
361tgacccccgc gccggcggtg attcccatcc tggtcatcgc ctgtgaccgc agcactgttc
421ggcgctgcct ggacaagctg ctgcattatc ggccctcggc tgagctcttc cccatcatcg
481ttagccagga ctgcgggcac gaggagacgg cccaggccat cgcctcctac ggcagcgcgg
541tcacgcacat ccggcagccc gacctgagca gcattgcggt gccgccggac caccgcaagt
601tccagggcta ctacaagatc gcgcgccact accgctgggc gctgggccag gtcttccggc
661agtttcgctt ccccgcggcc gtggtggtgg aggatgacct ggaggtggcc ccggacttct
721tcgagtactt tcgggccacc tatccgctgc tgaaggccga cccctccctg tggtgcgtct
781cggcctggaa tgacaacggc aaggagcaga tggtggacgc cagcaggcct gagctgctct
841accgcaccga ctttttccct ggcctgggct ggctgctgtt ggccgagctc tgggctgagc
901tggagcccaa gtggccaaag gccttctggg acgactggat gcggcggccg gagcagcggc
961aggggcgggc ctgcatacgc cctgagatct caagaacgat gacctttggc cgcaagggtg
1021tgagccacgg gcagttcttt gaccagcacc TCAAGTTTAT CAAGCTGAAC CAGCAGTTTG
1081TGCACTTCAC CCAGCTGGAC CTGTCTTACC TGCAGCGGGA GGCCTATGAC CGAGATTTCC
1141TCGCCCGCGT CTACGGTGCT CCCCAGCTGC AGGTGGAGAA AGTGAGGACC AATGACCGGA
1201AGGAGCTGGG GGAGGTGCGG GTGCAGTATA CGGGCAGGGA CAGCTTCAAG GCTTTCGCCA
1261AGGCTCTGGG TGTCATGGAT GACCTTAAGT CGGGGGTTCC GAGAGCTGGC TACCGGGGTA
1321TTGTCACCTT CCAGTTCCGG GGCCGCCGTG TCCACCTGGC GCCCCCACCG ACGTGGGAGG
1381GCTATGATCC TAGCTGGAAT TACCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC
1441TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA
1501AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGTAT CACGGCTATG
1561GGTACTGACA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)