Construct: ORF TRCN0000465609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003743.1_s317c1
- Derived from:
- ccsbBroadEn_13298
- DNA Barcode:
- AATAAATTTCGAACCAGTGAATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V0E2 (155066)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367791.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 2 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367792.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 3 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367793.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 4 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367794.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 5 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367795.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 6 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367796.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 7 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367797.1 | 27.1% | 14.3% | 1_196del;324_325ins148 |
| 8 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001367788.1 | 21.6% | 11.2% | 1_316del;444_445ins148 |
| 9 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001100592.2 | 16.2% | 8.3% | 1_511del;639_640ins148 |
| 10 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NM_001289990.1 | 14.9% | 7.6% | 1_580del;708_709ins148 |
| 11 | human | 155066 | ATP6V0E2 | ATPase H+ transporting V0 s... | NR_110612.1 | 9.7% | 1_1635del;1818A>G;1912_2808del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 366
- ORF length:
- 276
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCCTGCTC CAATACCCGC ACTGCTCTGG 121 AGTTTGCCCT CTTTCCCAAG GAGATGCTGC TGGGGAGCTG GTATGGGTGG GGTCTTTCCC 181 TTTACAGACG GGGCAGATGC CAGGACTCAG CCCATCCTGA GGAGGACACG TGTCCTCATG 241 GAGAGGGTGC TCCGGCCCAG GCGGGGGAGT CGGTGCCCAG TCAGCAGCTC TGCCACCATC 301 CTGCTGGGAA CTGGGGGGGC CTCTATTGGG TTATAGGCAA GGCCTTTTCT CTGGCATGGA 361 ATTGTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 421 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 481 CTTGGCTTTA TATATCTTGT GGAAAGGACG AAATAAATTT CGAACCAGTG AATATACGCG 541 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt