Construct: ORF TRCN0000465622
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014652.1_s317c1
- Derived from:
- ccsbBroadEn_10730
- DNA Barcode:
- TGCCTTCGGTTAAAAAAGCTCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDX1 (1044)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465622
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1044 | CDX1 | caudal type homeobox 1 | NM_001804.3 | 56.9% | 55% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 531
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgctggaca aggattcgcc cgggcacacc gtcctcgccc ggagcgcaga 121 ggcggacgcc cTACGAGTGG ATGCGGCGCA GCGTGGCGGC CGGAGGCGGC GGTGGCAGCG 181 GTAAGACTCG GACCAAGGAC AAGTACCGCG TGGTCTACAC CGACCACCAA CGCCTGGAGC 241 TGGAGAAGGA GTTTCATTAC AGCCGTTACA TCACAATCCG GCGGAAATCA GAGCTGGCTG 301 CCAATCTGGG GCTCACTGAA CGGCAGGTGA AGATCTGGTT CCAAAACCGG CGGGCAAAGG 361 AGCGCAAAGT GAACAAGAAG AAACAGCAGC AGCAACAGCC CCCACAGCCG CCGATGGCCC 421 ACGACATCAC GGCCACCCCA GCCGGGCCAT CCCTGGGGGG CCTGTGTCCC AGCAACACCA 481 GCCTCCTGGC CACCTCCTCT CCAATGCCTG TGAAAGAGGA GTTTCTGCCA TTGCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGATGCC TTCGGTTAAA AAAGCTCTCC ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt