Construct: ORF TRCN0000465645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001613.1_s317c1
- Derived from:
- ccsbBroadEn_00123
- DNA Barcode:
- ATATGAATTGTTTCGATTTCCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP1B2 (482)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 482 | ATP1B2 | ATPase Na+/K+ transporting ... | NM_001678.5 | 99.8% | 100% | 396A>G |
| 2 | human | 482 | ATP1B2 | ATPase Na+/K+ transporting ... | NM_001303263.1 | 71.6% | 71.7% | 0_1ins246;150A>G |
| 3 | mouse | 11932 | Atp1b2 | ATPase, Na+/K+ transporting... | NM_013415.5 | 90.9% | 97.2% | (many diffs) |
| 4 | mouse | 11932 | Atp1b2 | ATPase, Na+/K+ transporting... | XM_017314232.1 | 73.5% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 939
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtcatccag aaagagaaga agagctgcgg gcaggtggtt gaggagtgga 121 aggagttcgt gtggaacccg aggacgcacc agtttatggg ccgcaccggg accagctggg 181 cctttatcct cctcttctac ctcgtttttt atgggttcct caccgccatg ttcaccctca 241 ccatgtgggt gatgctgcag actgtctccg accatacccc caagtaccag gaccgactgg 301 ccacaccggg cttgatgatt cgccccaaga ctgagaacct tgatgtcatt gtcaatgtca 361 gtgacactga aagctgggac cagcatgttc agaagctcaa caagttcttg gagccttaca 421 acgactctat ccaagcccaa aagaatgatg tctgccgccc tgggcgctat tacgaacagc 481 cagataatgg agtcctcaac taccccaaac gtgcctgcca attcaaccgg acccagctgg 541 gcaactgctc cggcattggg gactccaccc actatggtta cagcactggg cagccctgtg 601 tcttcatcaa gatgaaccgg gtcatcaacT TCTATGCAGG AGCAAACCAG AGCATGAATG 661 TTACCTGTGC TGGGAAGCGA GATGAAGATG CTGAGAATCT CGGCAACTTC GTCATGTTCC 721 CCGCCAACGG CAACATCGAC CTCATGTACT TCCCCTACTA TGGCAAAAAG TTCCACGTGA 781 ACTACACACA GCCCCTGGTG GCTGTGAAGT TCCTGAATGT GACCCCCAAC GTGGAGGTGA 841 ATGTAGAATG TCGCATCAAC GCCGCCAACA TCGCCACAGA CGATGAGCGA GACAAGTTCG 901 CCGGCCGCGT GGCCTTCAAA CTCCGCATCA ACAAAACCTT GCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGAATATGA ATTGTTTCGA TTTCCCGCAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt