Construct: ORF TRCN0000465657
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008685.1_s317c1
- Derived from:
- ccsbBroadEn_11603
- DNA Barcode:
- GCTCACAGAACCAACCGTATTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BVES (11149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465657
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11149 | BVES | blood vessel epicardial sub... | NM_001199563.2 | 80.1% | 80% | 1_213del;1025A>G |
| 2 | human | 11149 | BVES | blood vessel epicardial sub... | NM_007073.4 | 80.1% | 80% | 1_213del;1025A>G |
| 3 | human | 11149 | BVES | blood vessel epicardial sub... | NM_147147.3 | 80.1% | 80% | 1_213del;1025A>G |
| 4 | human | 11149 | BVES | blood vessel epicardial sub... | XM_011535398.2 | 80.1% | 80% | 1_213del;1025A>G |
| 5 | mouse | 23828 | Bves | blood vessel epicardial sub... | NM_024285.2 | 68.4% | 71.1% | (many diffs) |
| 6 | mouse | 23828 | Bves | blood vessel epicardial sub... | XM_006512736.3 | 68.4% | 71.1% | (many diffs) |
| 7 | mouse | 23828 | Bves | blood vessel epicardial sub... | XM_006512737.2 | 68.4% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 933
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt aactctagga tgtacccttt atatcgtctg ggccactctc taccgatgtg 121 ccttggatat aatgatctgg aactctgtgt tcttgggtgt caacattttg catctgtcgt 181 atcttttata caagaagaga ccggtaaaga ttgaaaagga actcagtggc atgtaccggc 241 gattgtttga accactccgt gtgcctccag atttgttcag aagactaact ggacagtttt 301 gcatgatcca aaccttgaaa aagggccaaa cttatgctgc agaggataaa acctcagttg 361 atgaccgtct gagtattctc ttgaagggaa aaatgaaggt ctcctatcga ggacattttc 421 tgcataacat ttacccctgt gcctttatag attctcctga atttagatca actcagatgc 481 acaaaggtga aaaattccag gtcaccatta ttgcagatga taactgcaga tttttatgct 541 ggtcaagaga aagattaaca tactttctgg aatcagaacc tttcttgtat gaaatcttta 601 ggtatcttat tGGAAAAGAC ATCACAAATA AGCTCTACTC ATTGAATGAT CCCACCTTAA 661 ATGATAAAAA AGCCAAAAAG CTGGAACATC AGCTCAGCCT CTGCACACAG ATCTCCATGT 721 TGGAAATGAG GAACAGTATA GCCAGCTCCA GTGACAGTGA CGACGGCTTG CACCAGTTTC 781 TTCGGGGTAC CTCCAGCATG TCCTCTCTTC ATGTGTCATC CCCACACCAG CGAGCCTCTG 841 CCAAGATGAA ACCGATAGAA GAAGGAGCAG AAGATGGTGA TGACGTTTTT GAACCGGCAT 901 CTCCAAATAC ATTGAAAGTC CATCAGCTGC CTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 961 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1021 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGC 1081 TCACAGAACC AACCGTATTA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1141 ttgtgaaaga tt