Construct: ORF TRCN0000465659
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013271.1_s317c1
- Derived from:
- ccsbBroadEn_13294
- DNA Barcode:
- GTACCCTAGGTCAATGATCGGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3RF2 (153769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465659
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_005268380.2 | 29.8% | 29.6% | 1_1545del;1792T>C;2147G>C |
| 2 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_011537567.2 | 29.8% | 29.6% | 1_1545del;1792T>C;2147G>C |
| 3 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_011537568.2 | 29.8% | 29.6% | 1_1545del;1792T>C;2147G>C |
| 4 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | NM_152550.4 | 29% | 28.8% | (many diffs) |
| 5 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_006714757.1 | 29% | 28.8% | (many diffs) |
| 6 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_011537569.2 | 29% | 28.8% | (many diffs) |
| 7 | human | 153769 | SH3RF2 | SH3 domain containing ring ... | XM_017009136.1 | 29% | 28.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag aaagagtaag tggtggcaga gagatggatc cctgcagaga cccctccagt 121 ccgggatccc cactctcgtg gtaggctccc tcagacgcag ccccaccatg gtccttcggc 181 ctcagcagtt ccaattctac cagccacagg ggatcccctc ctccccctca gccgtggtgg 241 tggagatggg gtccaagcct gccctcacgg gggagcccgc cctcacgtgc atcagcaggg 301 gcagtgaggc ccggatccac tccgcggcca gctccctcat tatggaagac aaagaaatcc 361 ccatcaagag tgagcctctg ccaaaaccgc ccgcatctgc cccaccatcc atcctggtga 421 aaccagaaaa ctcaagaaat ggcatcgaaa agcaagtcaa aaccgtgaga tttcagaatt 481 acagccctcc tcccaccaaa cattacacct cccatcccac cTCCGGAAAG CCTGAACAGC 541 CAGCCACCCT CAAGGCGTCC CAGCCTGAAG CAGCGTCCTT GGGCCCAGAG ATGACCGTCC 601 TATTTGCCCA CCGAAGTGGC TGCCACTCCG GACAGCAGAC AGACCTCCGG AGAAAGTCAG 661 CTCTTGCCAA GGCCACAACC CTGGTGTCCA CTGCCTCAGG CACGCAGACC GTGTTTCCCA 721 GCAAATGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTACCCTAG GTCAATGATC GGCAGACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt