Construct: ORF TRCN0000465663
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014681.1_s317c1
- Derived from:
- ccsbBroadEn_04721
- DNA Barcode:
- TATCAAGGGATTTCACCCCGGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIA2 (117153)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465663
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_054024.4 | 100% | 100% | |
| 2 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XR_002957554.1 | 60.2% | (many diffs) | |
| 3 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449597.1 | 53.7% | 52.7% | (many diffs) |
| 4 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XR_002957555.1 | 49.9% | (many diffs) | |
| 5 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449596.1 | 47.2% | 46.3% | (many diffs) |
| 6 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449595.1 | 46.7% | 45.8% | (many diffs) |
| 7 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001329214.3 | 45.7% | 44.9% | (many diffs) |
| 8 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449594.1 | 45.6% | 44.7% | (many diffs) |
| 9 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449593.1 | 45.4% | 44.6% | (many diffs) |
| 10 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449592.1 | 45.3% | 44.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2031
- ORF length:
- 1962
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaaaattt ggcgttcaca gaatccttct tctggctatt tctctgacaa 121 agtgtctgga gagtacaaaa ctgctggcag accttaaaaa atgtggtgac ttggaatgtg 181 aagctttaat aaacagagtc tcagccatga gagattatag aggacctgac tgccgatacc 241 tgaacttcac taagggagaa gagatatctg tttatgttaa acttgcagga gaaagggaag 301 atttgtgggc aggaagtaaa ggaaaggagt ttggatattt tcccagagat gcagtccaga 361 ttgaagaggt gttcatatct gaggaaattc agatgtcaac gaaagaatct gactttcttt 421 gtcttcttgg agtaagttac acatttgaca atgaagatag tgaattaaac ggtgattatg 481 gtgaaaatat atatccttat gaagaagata aagatgaaaa atctagtata tatgaaagtg 541 attttcagat agaacctgga ttttatgcaa cttatgaaag tactttgttt gaagaccaag 601 ttccagcatt agaggctcct gaagatatcg gaagtaccag tgaatcaaaa gactgggaag 661 aagtagttgt tgaaagtatg gaacaggatc gtattccaga agtgcatgtc ccaccatctt 721 cagctgtgtc tggagtcaaa gaatggtttg gattgggagg agaacaagct gaagagaagg 781 cttttgaatc agttattgaa cctgtacaag aaagctcatt tcggagtaga aaaatagcag 841 tggaagatga gaatgaccta gaggaattaa ataatggtga gcctcaaaca gaacatcagc 901 aagaatctga atcagaaatt gattcagtgc caaagacaca gtctgaacta gcatctgagt 961 cagagcacat tcccaaacct caatccactg gttggtttgg tggaggattt acaagttatt 1021 taggttttgg agatgaggat acagggcttg aattaatagc tgaagaaagc aatccaccac 1081 tacaagattt tcccaattcc atatcatctg ataaagaagc cacagttcca tgtacagaaa 1141 tattaacaga aaaaaaagac acaatcacta atgatagctt gagtctcaag ccaagttggt 1201 ttgattttgg ttttgctata ctaggctttg catatgccaa ggaagataaa attatgttag 1261 atgacaggaa aaatgaagaa gatggtgggg cagatgaaca tgaacatcct ctaacaagtg 1321 aattagaccc tgaaaaagaa caagaaatag aaacgataaa aattatagaa acagaagatc 1381 aaatagacaa gaaaccagtc tcagaaaaaa cagacgaatc tgatactata ccatatttga 1441 aaaagttctt gtataatttt gacaaccctt ggaacttcca gaacattcca aaggaaacag 1501 aattgccatt tcccaaacag atactggatc aaaataatgt aattgaaaat gaagaaactg 1561 gagaattttc cattgataat tatcccacag ataatacaaa agttatgata ttcaaaagtt 1621 catacagtct gtcagatatg gtctctaaca tagagttacc tacgagaatt cacgaagaag 1681 tatattttga accctcatct tctaaagata gtgatgaaaa ttcgaaacca tCAGTAGACA 1741 CCGAAGGGCC TGCTCTGGTG GAGATAGACA GATCTGTGGA AAATACCCTG CTAAATAGTC 1801 AGATGGTTTC AACTGATAAC TCTTTGTCTT CTCAAAATTA TATTTCTCAG AAAGAAGATG 1861 CTTCTGAGTT TCAGATTCTG AAATACTTAT TCCAAATTGA TGTTTATGAT TTCATGAATT 1921 CTGCATTTTC ACCAATTGTA ATTCTTACAG AAAGGGTAAG TTTGCCTTTT AAACCTTTTG 1981 CAATAATTTT ACCTATTTTG CTAAATATAA GAGTGGCTAC AAAATATGTT TTGCCAACTT 2041 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2101 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2161 CTTGTGGAAA GGACGATATC AAGGGATTTC ACCCCGGACT ACGCGTTAAG TCgacaatca 2221 acctctggat tacaaaattt gtgaaagatt