Construct: ORF TRCN0000465687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002790.1_s317c1
- Derived from:
- ccsbBroadEn_09102
- DNA Barcode:
- ATGGATTGAAGTGGTTCCCTTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM47 (83604)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83604 | TMEM47 | transmembrane protein 47 | NM_031442.4 | 99.8% | 100% | 177G>C |
| 2 | mouse | 192216 | Tmem47 | transmembrane protein 47 | NM_138751.2 | 93% | 98.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttcggcgggc agcggcatgg aggaggtgcg cgtgtcggtg ctgaccccct 121 tgaagctggt cgggctggtg tgcatcttcc tggcgctgtg tctggacctg ggggcggtgc 181 tgagcccggc ctgggtcaca gctgaccacc agtactacct gtcgttgtgg gagtcctgcc 241 gcaaacccgc cagcttgGAC ATCTGGCACT GCGAGTCCAC GCTCAGCAGC GATTGGCAGA 301 TTGCTACTCT GGCTTTACTC CTGGGCGGCG CTGCCATCAT TCTCATTGCA TTCCTGGTGG 361 GTTTGATTTC TATCTGCGTG GGATCTCGAA GGCGTTTCTA TAGACCTGTT GCGGTCATGC 421 TTTTTGCAGC AGTTGTTTTA CAGGTTTGCA GCCTGGTCCT TTACCCAATC AAGTTCATTG 481 AAACTGTGAG CTTGAAAATT TACCATGAGT TCAACTGGGG TTATGGCCTG GCCTGGGGTG 541 CAACTATATT TTCGTTTGGG GGTGCCATCC TTTATTGCCT GAACCCTAAG AACTATGAAG 601 ACTACTACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATGGAT TGAAGTGGTT CCCTTGCAAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt