Construct: ORF TRCN0000465739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008859.1_s317c1
- Derived from:
- ccsbBroadEn_01529
- DNA Barcode:
- TTTGCAAGGTCCGTGACCGAGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3BGRL (6451)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6451 | SH3BGRL | SH3 domain binding glutamat... | NM_003022.3 | 100% | 100% | |
| 2 | human | 6451 | SH3BGRL | SH3 domain binding glutamat... | XM_011531014.1 | 73.9% | 65.5% | (many diffs) |
| 3 | human | 6451 | SH3BGRL | SH3 domain binding glutamat... | XM_011531013.1 | 63.4% | 56.2% | (many diffs) |
| 4 | mouse | 56726 | Sh3bgrl | SH3-binding domain glutamic... | NM_019989.2 | 92.6% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgGCATGGT GATCCGTGTA TATATTGCAT CTTCCTCTGG CTCTACAGCG ATTAAGAAGA 121 AACAACAAGA TGTGCTTGGT TTCCTAGAAG CCAACAAAAT AGGATTTGAA GAAAAAGATA 181 TTGCAGCCAA TGAAGAGAAT CGGAAGTGGA TGAGAGAAAA TGTACCTGAA AATAGTCGAC 241 CAGCCACAGG TTACCCCCTG CCACCTCAGA TTTTCAATGA AAGCCAGTAT CGCGGGGACT 301 ATGATGCCTT CTTTGAAGCC AGAGAAAATA ATGCAGTGTA TGCCTTCTTA GGCTTGACAG 361 CCCCACCTGG TTCAAAGGAA GCAGAAGTGC AAGCAAAGCA GCAAGCATGC CCCAACTTTC 421 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 481 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 541 TGTGGAAAGG ACGATTTGCA AGGTCCGTGA CCGAGTTCAC GCGTTAAGTC gacaatcaac 601 ctctggatta caaaatttgt gaaagatt