Construct: ORF TRCN0000465765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001491.1_s317c1
- Derived from:
- ccsbBroadEn_04028
- DNA Barcode:
- CTTATAACTCTGCTTACGCATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C7orf25 (79020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465765
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79020 | C7orf25 | chromosome 7 open reading f... | NM_001363436.1 | 100% | 100% | |
| 2 | human | 79020 | C7orf25 | chromosome 7 open reading f... | NM_024054.3 | 100% | 100% | |
| 3 | human | 79020 | C7orf25 | chromosome 7 open reading f... | XM_017012597.2 | 100% | 100% | |
| 4 | human | 79020 | C7orf25 | chromosome 7 open reading f... | XM_017012596.1 | 96.7% | 96.7% | 1_42del |
| 5 | human | 79020 | C7orf25 | chromosome 7 open reading f... | NM_001099858.1 | 87.8% | 87.8% | 1_174del |
| 6 | mouse | 105351 | AW209491 | expressed sequence AW209491 | NM_001104646.1 | 88.1% | 92.6% | (many diffs) |
| 7 | mouse | 105351 | AW209491 | expressed sequence AW209491 | NM_134067.4 | 88.1% | 92.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcacattcc atgctctgtg aacgaatcgc catagccaag gaactgatca 121 agagagcaga atcactttct agatcaagaa aaggtggcat agaaggtggt gcaaagctgt 181 gcagcaaatt gaaggcagaa ttaaaattct tgcagaaagt agaagctggg aaagtagcta 241 ttaaagagtc tcatttacag agcactaacc taacacacct gagagccatt gtggaatcag 301 cagaaaacct ggaagaagtt gttagtgttc ttcatgtctt tggttataca gataccttag 361 gagaaaagca aacccttgtg gtagatgtag ttgcaaatgg tggtcatact tgggtgaaag 421 ccattggccg gaaggctgaa gctcttcata acatctggct gggcaggggc caatatggtg 481 acaaaagcat cattgagcag gctgaagact tcctccaggc cagtcaccag cagccagtgc 541 agtatagcaa ccctcacatc atctttgcat tttacaacag tgtctccagc cccatggcag 601 agaagctgaa agaaatgggc atatctgtga gaggagacat agtagcagtc aacgctctgt 661 tagatcaccc tgaagagctt caaccaagtg agagtgaatc agatgatgag ggccctgaac 721 ttttgcaggt gaccagagtt gaccgagaaa atatactagc aagtgttgcg tttccaacag 781 aaattaaggt cgatgtgtgc aaaagagtaa atctggacat tactacttta atcacatatg 841 tatctgccct cagctatgga ggctgccact ttattttcaa agagaaagtg ctcacagaac 901 aagcagagca agagaggaaa gagcaggttc tacctcagct ggaggccttt atgaaggaca 961 aggagttgtt tgcttgtgaa tctgctgtca aggactttca gtctatttta gataccttag 1021 gaggacctgg ggagagagag agggccactg tgttaattaa gcgaattaat gtggtaccag 1081 accagccttc tgagcgtgcc ttgagactag tggccagttc aaaaattaat agccgctcat 1141 taacaatttt tgggacggga gacaccctaa aagccatcac aatgactgct aATAGTGGTT 1201 TTGTCAGAGC TGCAAACAAC CAGGGTGTTA AATTTAGTGT GTTTATCCAT CAGCCCAGAG 1261 CACTTACTGA GAGTAAAGAG GCTCTAGCCA CCCCCTTACC AAAAGACTAC ACAACTGACA 1321 GTGAACACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTATA ACTCTGCTTA CGCATACAAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt