Construct: ORF TRCN0000465767
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011046.1_s317c1
- Derived from:
- ccsbBroadEn_12365
- DNA Barcode:
- CTTCAACTGATCTACACCTCTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLHL14 (57565)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465767
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57565 | KLHL14 | kelch like family member 14 | XM_011526109.2 | 93.8% | 92.5% | (many diffs) |
| 2 | human | 57565 | KLHL14 | kelch like family member 14 | NM_020805.3 | 59.7% | 57.5% | (many diffs) |
| 3 | mouse | 225266 | Klhl14 | kelch-like 14 | NM_001081403.1 | 56.3% | 57% | (many diffs) |
| 4 | mouse | 225266 | Klhl14 | kelch-like 14 | XM_006525822.2 | 56.3% | 57% | (many diffs) |
| 5 | mouse | 225266 | Klhl14 | kelch-like 14 | XM_006525823.3 | 56.3% | 57% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagatccggg gacaggacct ccaccttcga ccccagccac agcgacaacc 121 tgctgcacgg cctcaacctg ctgtggagga agcagctgtt ttgcgacgtg accctgacgg 181 cccagggcca gcagttccat tgccacaagg ccgtgctggc ctcctgctcg cagtacttcc 241 gatcgctctt ctccagccac ccccctctcg ggggaggggt cggcggccag gacggcctgg 301 gggcccccaa ggaccagcag cagccgccgc agcagcagcc gtcacagcag cagcagccgc 361 cgccgcagga ggagcccggg actccttctt cctcccccga cgacaagctg ctgaccagcc 421 cccgggccat caacaacctg gtgctgcagg gctgctcgtc catcgggctg cgcctggtgc 481 tcgagtacct ctacacggcc aacgtgaccc tgtccctgga cacggtggag gaggtgctgt 541 cggtcagcaa gatcctgcac atcccccagg tcaccaagct ctgcgtgcag ttcctcaacg 601 accagatctc ggtgcagaac tacaagcagg tgtgcaagat cgccgcgctg cacggcctgg 661 aggagaccaa gaagctggcc aacaagtacc tggtggagga tgtgctgctg ctcaacttcg 721 aggagatgcg cgccctgctg gactcgctgc cgccccccgt ggagtcggag ctggcgctct 781 tccagatgtc cgtgctgtgg ctggagcacg accgcgagac ccgcatgcag tatgcgcctg 841 acctcatgaa gcgccTCCGC TTCGCCCTCA TCCCGGCCCC GGAGCTGGTG GAGCGGGTCC 901 AGTCAGTGGA TTTCATGCGA ACCGACCCAG TCTGCCAGAA GCTGCTGCTG GACGCCATGA 961 ACTACCACCT GATGCCCTTC AGGCAGCACT GCAGGCAGAG CCTGGCCAGC AGAATTCGCT 1021 CTAACAAGAA AATGCTGTTA TTGGTTGGAG GGCTGCCTCC TGGACCGGAC CGGCTCCCCA 1081 GCAATTTGGT TCAGTATTAC GACGATGAAA AGAAGACATG GAAAATACTC ACAATCACAG 1141 GACCACACCC CATTTCAGGG AAGCAGGAAA GTGCAAGCTC ACCAGAGTTC AGAAGGAGGA 1201 AAACCAGAGA ATTTTCAAAC AACACAATGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTTCA 1381 ACTGATCTAC ACCTCTTATA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt