Construct: ORF TRCN0000465781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000339.1_s317c1
- Derived from:
- ccsbBroadEn_09828
- DNA Barcode:
- CCCGCTCTGTGTCGGCCCGTCTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC25A43 (203427)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203427 | SLC25A43 | solute carrier family 25 me... | NM_145305.3 | 99.9% | 99.7% | 1001C>T |
| 2 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XM_017029340.1 | 73.1% | 64.9% | (many diffs) |
| 3 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XM_017029341.1 | 67.7% | 67.4% | 687_688insCAGGCT;693_694ins324 |
| 4 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_001755664.1 | 66.9% | (many diffs) | |
| 5 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_938547.1 | 65% | 1_78del;767_852del;936_937ins251 | |
| 6 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_938545.1 | 60.5% | 1_78del;768_940del;1022_1023ins252 | |
| 7 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_001755662.1 | 60.3% | 1_78del;767_945del;1029_1030ins251 | |
| 8 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_938546.1 | 57.5% | 1_78del;766_1097del;1235_1236ins198 | |
| 9 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_001755663.1 | 39% | (many diffs) | |
| 10 | human | 203427 | SLC25A43 | solute carrier family 25 me... | XR_001755661.1 | 38.1% | (many diffs) | |
| 11 | mouse | 194744 | Slc25a43 | solute carrier family 25, m... | NM_001085497.2 | 85.4% | 85.9% | (many diffs) |
| 12 | mouse | 194744 | Slc25a43 | solute carrier family 25, m... | NR_075096.1 | 69.9% | (many diffs) | |
| 13 | mouse | 194744 | Slc25a43 | solute carrier family 25, m... | XM_006541379.2 | 59.3% | 57.4% | (many diffs) |
| 14 | mouse | 194744 | Slc25a43 | solute carrier family 25, m... | XM_006541381.3 | 58.6% | 58.6% | (many diffs) |
| 15 | mouse | 194744 | Slc25a43 | solute carrier family 25, m... | XM_006541380.3 | 58.6% | 58.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1089
- ORF length:
- 1023
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacgtggagg cgggacggcc gactgacagg cggccaaagg ctgctgtgcg 121 ctgggctggc ggggacgctc agcctcagcc tcaccgcgcc cctggagctc gccaccgtgc 181 tggcccaggt tggcgtcgtg cgaggccacg cccggggacc gtgggccaca gggcaccggg 241 tgtggcgggc agaggggctc cgggccctgt ggaaggggaa cgcggtggcg tgcctgcgcc 301 tcttcccctg cagcgccgtg cagctcgccg cctaccgcaa atttgttgtg ctgttcacag 361 atgacctggg ccacatttcc cagtggagct ccatcatggc tgggagtctc gcaggcatgg 421 tttccaccat tgtaacatat cctacagacc tcatcaaaac ccggttgatc atgcagaaca 481 tactggaacc atcgtacagg gggctcctcc atgctttttc tactatttac caacaggaag 541 ggttccttgc cctttatcga ggggtttccc tcactgttgt aggtgctctc ccgttctctg 601 ctggctccct tcttgtttac atgaacctgg agaaaatctg gaacggaccc cgagatcagt 661 tctctctccc acagaacttt gctaatgtct gtctggctgc tgcagtgacc cagaccctct 721 cctttccctt tgagaccgtg aagagaaaga tgcaggctca gagcccctac ctcccacaca 781 gtggaggagt agatgtccat ttctcaggag cagtggactg cttccggcag atagtgaagg 841 cccagggggt cctggggctc tggaatgGAT TGACAGCCAA TTTACTGAAG ATAGTTCCAT 901 ATTTTGGAAT TATGTTTAGC ACCTTTGAGT TCTGCAAGAG AATCTGTCTT TATCAAAATG 961 GTTACATTCT GTCTCCACTG AGCTATAAAT TGACCCCAGG AGTCGATCAG AGTTTGCAGC 1021 CCCAGGAATT ACGAGAATTA AAGAAGTTCT TCAAAACGAG AAAACTGAAG CCTAAAAAAC 1081 CAACTCTATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1141 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1201 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCCGCT CTGTGTCGGC CCGTCTACAC 1261 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt