Construct: ORF TRCN0000465794
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016996.1_s317c1
- Derived from:
- ccsbBroadEn_00153
- DNA Barcode:
- CGTCGTATTTGTTCATTACAGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCHE (590)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465794
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 590 | BCHE | butyrylcholinesterase | NM_000055.4 | 100% | 100% | |
| 2 | human | 590 | BCHE | butyrylcholinesterase | NR_137636.2 | 72.7% | 1_118del;1803_1881del;2004_2484del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1872
- ORF length:
- 1806
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tagcaaagtc acaatcatat gcatcagatt tctcttttgg tttcttttgc 121 tctgcatgct tattgggaag tcacatactg aagatgacat cataattgca acaaagaatg 181 gaaaagtcag agggatgaac ttgacagttt ttggtggcac ggtaacagcc tttcttggaa 241 ttccctatgc acagccacct cttggtagac ttcgattcaa aaagccacag tctctgacca 301 agtggtctga tatttggaat gccacaaaat atgcaaattc ttgctgtcag aacatagatc 361 aaagttttcc aggcttccat ggatcagaga tgtggaaccc aaacactgac ctcagtgaag 421 actgtttata tctaaatgta tggattccag cacctaaacc aaaaaatgcc actgtattga 481 tatggattta tggtggtggt tttcaaactg gaacatcatc tttacatgtt tatgatggca 541 agtttctggc tcgggttgaa agagttattg tagtgtcaat gaactatagg gtgggtgccc 601 taggattctt agctttgcca ggaaatcctg aggctccagg gaacatgggt ttatttgatc 661 aacagttggc tcttcagtgg gttcaaaaaa atatagcagc ctttggtgga aatcctaaaa 721 gtgtaactct ctttggagaa agtgcaggag cagcttcagt tagcctgcat ttgctttctc 781 ctggaagcca ttcattgttc accagagcca ttctgcaaag tggatccttt aatgctcctt 841 gggcggtaac atctctttat gaagctagga acagaacgtt gaacttagct aaattgactg 901 gttgctctag agagaatgag actgaaataa tcaagtgtct tagaaataaa gatccccaag 961 aaattcttct gaatgaagca tttgttgtcc cctatgggac tcctttgtca gtaaactttg 1021 gtccgaccgt ggatggtgat tttctcactg acatgccaga catattactt gaacttggac 1081 aatttaaaaa aacccagatt ttggtgggtg ttaataaaga tgaagggaca gcttttttag 1141 tctatggtgc tcctggcttc agcaaagata acaatagtat cataactaga aaagaatttc 1201 aggaaggttt aaaaatattt tttccaggag tgagtgagtt tggaaaggaa tccatccttt 1261 ttcattacac agactgggta gatgatcaga gacctgaaaa ctaccgtgag gccttgggtg 1321 atgttgttgg ggattataat ttcatatgcc ctgccttgga gttcaccaag aagttctcag 1381 aatggggaaa taatgccttt ttctactatt ttgaacaccg atcctccaaa cttccgtggc 1441 cagaatggat gggagtgatg catGGCTATG AAATTGAATT TGTCTTTGGT TTACCTCTGG 1501 AAAGAAGAGA TAATTACACA AAAGCCGAGG AAATTTTGAG TAGATCCATA GTGAAACGGT 1561 GGGCAAATTT TGCAAAATAT GGGAATCCAA ATGAGACTCA GAACAATAGC ACAAGCTGGC 1621 CTGTCTTCAA AAGCACTGAA CAAAAATATC TAACCTTGAA TACAGAGTCA ACAAGAATAA 1681 TGACGAAACT ACGTGCTCAA CAATGTCGAT TCTGGACATC ATTTTTTCCA AAAGTCTTGG 1741 AAATGACAGG AAATATTGAT GAAGCAGAAT GGGAGTGGAA AGCAGGATTC CATCGCTGGA 1801 ACAATTACAT GATGGACTGG AAAAATCAAT TTAACGATTA CACTAGCAAG AAAGAAAGTT 1861 GTGTGGGTCT CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1921 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1981 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT CGTATTTGTT CATTACAGAT 2041 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t