Construct: ORF TRCN0000465802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000511.1_s317c1
- Derived from:
- ccsbBroadEn_06078
- DNA Barcode:
- TAGGGGTGAACGCGAGCCTTCACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP19A1 (1588)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465802
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_000103.4 | 99.8% | 99.8% | 240A>G;619C>T |
| 2 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347248.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 3 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347249.2 | 99.8% | 99.8% | 240A>G;619C>T |
| 4 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347250.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 5 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347251.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 6 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347252.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 7 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347253.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 8 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347254.1 | 99.8% | 99.8% | 240A>G;619C>T |
| 9 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347255.2 | 99.8% | 99.8% | 240A>G;619C>T |
| 10 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_001347256.2 | 99.8% | 99.8% | 240A>G;619C>T |
| 11 | human | 1588 | CYP19A1 | cytochrome P450 family 19 s... | NM_031226.3 | 99.8% | 99.8% | 240A>G;619C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1575
- ORF length:
- 1509
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt tttggaaatg ctgaacccga tacattataa catcaccagc atcgtgcctg 121 aagccatgcc tgctgccacc atgccagtcc tgctcctcac tggccttttt ctcttggtgt 181 ggaattatga gggcacatcc tcaataccag gtcctggcta ctgcatggga attggacccc 241 tcatctccca cggcagattc ctgtggatgg ggatcggcag tgcctgcaac tactacaacc 301 gggtgtatgg agaattcatg cgagtctgga tctctggaga ggaaacactc attatcagca 361 agtcctcaag tatgttccac ataatgaagc acaatcatta cagctctcga ttcggcagca 421 aacttgggct gcagtgcatc ggtatgcatg agaaaggcat catatttaac aacaatccag 481 agctctggaa aacaactcga cccttcttta tgaaagctct gtcaggcccc ggccttgttc 541 gtatggtcac agtctgtgct gaatccctca aaacacatct ggacaggttg gaggaggtga 601 ccaatgaatc gggctatgtg gacgtgttga cccttctgcg tcgtgtcatg ctggacacct 661 ctaacacgct cttcttgagg atctctttgg acgaaagtgc tatcgtggtt aaaatccaag 721 gttattttga tgcatggcaa gctctcctca tcaaaccaga catcttcttt aagatttctt 781 ggctatacaa aaagtatgag aagtctgtca aggatttgaa agatgccata gaagttctga 841 tagcagaaaa aagacgcagg atttccacag aagagaaact ggaagaatgt atggactttg 901 ccactgagtt gattttagca gagaaacgtg gtgacctgac aagagagaat gtgaaccagt 961 gcatattgga aatgctgatc gcagctcctg acaccatgtc tgtctctttg ttcttcatgc 1021 tatttctcat tgcaaagcac cctaatgttg aagaggcaat aataaaggaa atccagactg 1081 ttattggtga gagagacata aagattgatg atatacaaaa attaaaagtg atggaaaact 1141 tcatttatga gagcatgcgg taccagcctg tcgtggactt ggtcatgcgc aaagccTTAG 1201 AAGATGATGT AATCGATGGC TACCCAGTGA AAAAGGGGAC AAACATTATC CTGAATATTG 1261 GAAGGATGCA CAGACTCGAG TTTTTCCCCA AACCCAATGA ATTTACTCTT GAAAATTTTG 1321 CAAAGAATGT TCCTTATAGG TACTTTCAGC CATTTGGCTT TGGGCCCCGT GGCTGTGCAG 1381 GAAAGTACAT CGCCATGGTG ATGATGAAAG CCATCCTCGT TACACTTCTG AGACGATTCC 1441 ACGTGAAGAC ATTGCAAGGA CAGTGTGTTG AGAGCATACA GAAGATACAC GACTTGTCCT 1501 TGCACCCAGA TGAGACTAAA AACATGCTGG AAATGATCTT TACCCCAAGA AACTCAGACA 1561 GGTGTCTGGA ACACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1621 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1681 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGGGGTGAA CGCGAGCCTT 1741 CACGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt