Construct: ORF TRCN0000465802

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000511.1_s317c1
Derived from:
ccsbBroadEn_06078
DNA Barcode:
TAGGGGTGAACGCGAGCCTTCACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CYP19A1 (1588)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465802
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_000103.4 99.8% 99.8% 240A>G;619C>T
2 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347248.1 99.8% 99.8% 240A>G;619C>T
3 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347249.2 99.8% 99.8% 240A>G;619C>T
4 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347250.1 99.8% 99.8% 240A>G;619C>T
5 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347251.1 99.8% 99.8% 240A>G;619C>T
6 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347252.1 99.8% 99.8% 240A>G;619C>T
7 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347253.1 99.8% 99.8% 240A>G;619C>T
8 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347254.1 99.8% 99.8% 240A>G;619C>T
9 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347255.2 99.8% 99.8% 240A>G;619C>T
10 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_001347256.2 99.8% 99.8% 240A>G;619C>T
11 human 1588 CYP19A1 cytochrome P450 family 19 s... NM_031226.3 99.8% 99.8% 240A>G;619C>T
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1575
ORF length:
1509
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt tttggaaatg ctgaacccga tacattataa catcaccagc atcgtgcctg
121aagccatgcc tgctgccacc atgccagtcc tgctcctcac tggccttttt ctcttggtgt
181ggaattatga gggcacatcc tcaataccag gtcctggcta ctgcatggga attggacccc
241tcatctccca cggcagattc ctgtggatgg ggatcggcag tgcctgcaac tactacaacc
301gggtgtatgg agaattcatg cgagtctgga tctctggaga ggaaacactc attatcagca
361agtcctcaag tatgttccac ataatgaagc acaatcatta cagctctcga ttcggcagca
421aacttgggct gcagtgcatc ggtatgcatg agaaaggcat catatttaac aacaatccag
481agctctggaa aacaactcga cccttcttta tgaaagctct gtcaggcccc ggccttgttc
541gtatggtcac agtctgtgct gaatccctca aaacacatct ggacaggttg gaggaggtga
601ccaatgaatc gggctatgtg gacgtgttga cccttctgcg tcgtgtcatg ctggacacct
661ctaacacgct cttcttgagg atctctttgg acgaaagtgc tatcgtggtt aaaatccaag
721gttattttga tgcatggcaa gctctcctca tcaaaccaga catcttcttt aagatttctt
781ggctatacaa aaagtatgag aagtctgtca aggatttgaa agatgccata gaagttctga
841tagcagaaaa aagacgcagg atttccacag aagagaaact ggaagaatgt atggactttg
901ccactgagtt gattttagca gagaaacgtg gtgacctgac aagagagaat gtgaaccagt
961gcatattgga aatgctgatc gcagctcctg acaccatgtc tgtctctttg ttcttcatgc
1021tatttctcat tgcaaagcac cctaatgttg aagaggcaat aataaaggaa atccagactg
1081ttattggtga gagagacata aagattgatg atatacaaaa attaaaagtg atggaaaact
1141tcatttatga gagcatgcgg taccagcctg tcgtggactt ggtcatgcgc aaagccTTAG
1201AAGATGATGT AATCGATGGC TACCCAGTGA AAAAGGGGAC AAACATTATC CTGAATATTG
1261GAAGGATGCA CAGACTCGAG TTTTTCCCCA AACCCAATGA ATTTACTCTT GAAAATTTTG
1321CAAAGAATGT TCCTTATAGG TACTTTCAGC CATTTGGCTT TGGGCCCCGT GGCTGTGCAG
1381GAAAGTACAT CGCCATGGTG ATGATGAAAG CCATCCTCGT TACACTTCTG AGACGATTCC
1441ACGTGAAGAC ATTGCAAGGA CAGTGTGTTG AGAGCATACA GAAGATACAC GACTTGTCCT
1501TGCACCCAGA TGAGACTAAA AACATGCTGG AAATGATCTT TACCCCAAGA AACTCAGACA
1561GGTGTCTGGA ACACTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
1621CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
1681TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAGGGGTGAA CGCGAGCCTT
1741CACGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)