Construct: ORF TRCN0000465813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018296.1_s317c1
- Derived from:
- ccsbBroadEn_05429
- DNA Barcode:
- GTTACTCAGGCAAATCTAAGTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP19-1 (337882)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 337882 | KRTAP19-1 | keratin associated protein ... | NM_181607.3 | 100% | 100% | |
| 2 | human | 337970 | KRTAP19-3 | keratin associated protein ... | NM_181609.4 | 83.7% | 83.3% | (many diffs) |
| 3 | human | 337972 | KRTAP19-5 | keratin associated protein ... | NM_181611.3 | 70% | 63.3% | (many diffs) |
| 4 | human | 337974 | KRTAP19-7 | keratin associated protein ... | NM_181614.2 | 60.3% | 54.4% | (many diffs) |
| 5 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_181612.3 | 56.2% | 53.3% | (many diffs) |
| 6 | human | 337973 | KRTAP19-6 | keratin associated protein ... | NM_001303120.1 | 43.3% | 40.5% | (many diffs) |
| 7 | mouse | 105246181 | Gm41515 | predicted gene, 41515 | XM_011246170.1 | 78.5% | 74.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 363
- ORF length:
- 270
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGAGTCA CTACGGCAGC TACTACGGAG 121 GCCTGGGCTA CAGCTGTGGA GGCTTCGGTG GCCTGGGCTA TGGCTATGGC TGTGGATGTG 181 GCAGCTTCTG CAGACGGGGT TCTGGCTGTG GCTATGGAGG CTACGGATAT GGCTCTGGCT 241 TTGGAAGCTA CGGATATGGC TCTGGCTTTG GAGGCTACGG ATATGGCTCT GGCTTTGGAG 301 GCTATGGATA TGGCTGCTGC CGCCCATCGT ACAATGGAGG ATACGGATTC TCTGGCTTTT 361 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGAGT TACTCAGGCA AATCTAAGTT ATACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt