Construct: ORF TRCN0000465825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016230.1_s317c1
- Derived from:
- ccsbBroadEn_05286
- DNA Barcode:
- GCGTCCCAGAGCGCATCTACATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPESP1 (246777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 246777 | SPESP1 | sperm equatorial segment pr... | NM_145658.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcccttagtc cttctagttg cgcttttgct atggccttcg tctgtgccgg 121 cttatccgag cataactgtg acacctgatg aagagcaaaa cttgaatcat tatatacaag 181 ttttagagaa cctagtacga agtgttccct ctggggagcc aggtcgtgag aaaaaatcta 241 actctccaaa acatgtttat tctatagcat caaagggatc aaaatttaag gagctagtta 301 cacatggaga cgcttcaact gagaatgatg ttttaaccaa tcctatcagt gaagaaacta 361 caactttccc tacaggaggc ttcacaccgg aaataggaaa gaaaaaacac acggaaagta 421 ccccattctg gtcgatcaaa ccaaacaatg tttccattgt tttgcatgca gaggaacctt 481 atattgaaaa tgaagagcca gagccagagc cggagccagc tgcaaaacaa actgaggcac 541 caagaatgtt gccagttgtt actgaatcat ctacaagtcc atatgttacc tcatacaagt 601 cacctgtcac cactttagat aagagcactg gcattgggat ctctacagaa tcagaagatg 661 ttcctcagct ctcaggtgaa actgcgatag aaaaacccga agagtttgga aagcacccag 721 agagttggaa taatgatgac attttGAAAA AAATTTTAGA TATTAATTCA CAAGTGCAAC 781 AGGCACTTCT TAGTGACACC AGCAACCCAG CATATAGAGA AGATATTGAA GCCTCTAAAG 841 ATCACCTAAA ACGAAGCCTT GCTCTAGCAG CAGCAGCAGA ACATAAATTA AAAACAATGT 901 ATAAGTCCCA GTTATTGCCA GTAGGACGAA CAAGTAATAA AATTGATGAC ATCGAAACTG 961 TTATTAACAT GCTGTGTAAT TCTAGATCTA AACTCTATGA ATATTTAGAT ATTAAATGTG 1021 TTCCACCAGA GATGAGAGAA AAAGCTGCTA CAGTATTCAA TACATTAAAA AATATGTGTA 1081 GATCAAGGAG AGTCACAGCC TTATTAAAAG TTTATTACCC AACTTTCTTG TACAAAGTGG 1141 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1201 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1261 AGCGTCCCAG AGCGCATCTA CATGGACGCG TTAAGTCgac aatcaacctc tggattacaa 1321 aatttgtgaa agatt