Construct: ORF TRCN0000465826
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016276.1_s317c1
- Derived from:
- ccsbBroadEn_07927
- DNA Barcode:
- GCACAAGACTGCGTGCGGCTCAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSPT2 (23708)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465826
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23708 | GSPT2 | G1 to S phase transition 2 | NM_018094.5 | 99.7% | 99.5% | (many diffs) |
| 2 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_002094.4 | 84.9% | 85.2% | (many diffs) |
| 3 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_001130006.2 | 84.8% | 85.1% | (many diffs) |
| 4 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_001130007.2 | 71.4% | 74.2% | (many diffs) |
| 5 | mouse | 14853 | Gspt2 | G1 to S phase transition 2 | NM_008179.2 | 87.1% | 88.6% | (many diffs) |
| 6 | mouse | 14852 | Gspt1 | G1 to S phase transition 1 | NM_146066.2 | 82.8% | 85.2% | (many diffs) |
| 7 | mouse | 14852 | Gspt1 | G1 to S phase transition 1 | NM_001130008.1 | 82.6% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1950
- ORF length:
- 1884
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttcgggtagc agcagcagcg actcggcgcc cgattgctgg gaccaggtgg 121 acatggaatc cacggggtcg gccccgagcg gggatggagt ctcctctgcg gtggccgagg 181 cccagcgcga gcccctcagc tcggctttca gccgtaagct caacgtcaac gccaagccct 241 tcgtgcctaa cgtacacgcc gcggagttcg tgccgtcctt cctgcggggc ccgactcagc 301 cgcccaccct cccggccggc tccggcagca acgatgaaac ctgcaccggc gcgggatacc 361 ctcaaggtaa aaggatggga cggggggcac ctgtggaacc ttcccgagag gaaccgttag 421 tgtcgcttga aggttccaat tcagccgtta ccatggaact ttcagaacct gttgtagaaa 481 atggagaggt ggaaatggcc ctagaagaat catgggagca cagtaaagaa gtaagtgaag 541 ccgagcctgg gggtggttcc tcgggagatt cagggccccc agaagaaagt ggccaggaaa 601 tgatggagga aaaagaggaa ataagaaaat ccaaatctgt gatcgtaccc tcaggtgcac 661 ctaagaaaga acacgtaaat gtagtattca ttggccatgt agacgctggc aagtcaacca 721 tcggaggaca gataatgttt ttgactggaa tggttgacaa aagaacactg gagaaatatg 781 aaagagaagc taaggaaaaa aacagagaaa cctggtattt gtcctgggcc ttagatacaa 841 atcaggagga acgagacaag ggtaaaacag tcgaagtggg tcgtgcctat tttgaaacag 901 aaaggaaaca tttcacaatt ttagatgccc ctggccacaa gagttttgtc ccaaatatga 961 ttggtggtgc ttctcaagct gatttggctg tgctggtcat ctctgccagg aaaggagagt 1021 ttgaaactgg atttgaaaaa ggtggacaga caagagaaca tgcgatgttg gcaaaaacgg 1081 caggggtaaa acatttaata gtgcttatta ataagatgga tgatcacaca gtaaattgga 1141 gcatcgagag atatgaagaa tgtaaagaaa aactggtgcc ctttttgaaa aaagtaggct 1201 tcagtccaaa aaaggacatt cactttatgc cctgctcagg actgaccgga gcaaatatta 1261 aagagcagtc agatttctgc ccttggtaca ctggattacc atttattccg tatttggata 1321 acttgccaaa cttcaacaga tcaattgatg gaccaataag actgccaatt gtggataagt 1381 acaaagatat gggcactgtg gtcctgggaa agctggaatc cgggtccatt tttaaaggcc 1441 agcagctcgt gatgatgcca aacaagcaca atgtagaagt tcttggaata ctttctgatg 1501 atactgaaac tgattttgta gccccaggtg aaaacctcaa aatcagactg aagggaattg 1561 aagaagaaga gattcttcca ggattcatac tttgtgatcc tagtaaccTC TGCCATTCTG 1621 GACGCACGTT TGATGTTCAG ATAGTGATTA TTGAGCACAA ATCCATCATC TGCCCAGGTT 1681 ATAATGCGGT GCTGCACATT CATACTTGTA TTGAGGAAGT TGAGATAACA GCGTTAATCT 1741 CCTTGGTAGA CAAAAAATCA GGAGAAAAAA GTAAGACACG ACCCCGCTTC GTGAAACAAG 1801 ATCAAGTATG CATTGCTCGT TTAAGGACAG CAGGAACCAT CTGCCTCGAG ACGTTCAAAG 1861 ATTTTCCTCA GATGGGTCGT TTTACTTTAA GAGATGAGGG TAAGACCATT GCAATTGGAA 1921 AAGTTCTGAA ATTGGTCCAA GAGAAGGACT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1981 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 2041 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCACA 2101 AGACTGCGTG CGGCTCAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2161 tgaaagatt