Construct: ORF TRCN0000465830
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002999.1_s317c1
- Derived from:
- ccsbBroadEn_13718
- DNA Barcode:
- AAGGACGGCCAGCCGTTGAGTTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERICH1-AS1 (619343)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465830
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9228 | DLGAP2 | DLG associated protein 2 | NR_073397.2 | 30.2% | 1_285del;538_834del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 342
- ORF length:
- 252
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGGGACTGC TCAGGTTTTG CCTGGCATTC 121 TGCAGAAGCA TTGCTGTATC TTACCAGACA GGAATACAGG CCGTCTCCAA ACTATAGTCC 181 TATTGGACTG GTGCACCAAA TATGGATTTG GGGTGTGCGT AATTCAGTCC ATAGCACCCA 241 CCAAACCACA GAAGCTGCCC CGAGCTGATG TCCCGCTATC GGTGCGGCTG CAGGACACTG 301 TCCCATTGCT GCATGGAGCC TCTCGCTGTC TGAGCCTCAC CTGCCCAACT TTCTTGTACA 361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 481 AGGACGAAAG GACGGCCAGC CGTTGAGTTG GACGCGTTAA GTCgacaatc aacctctgga 541 ttacaaaatt tgtgaaagat t