Construct: ORF TRCN0000465837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012361.1_s317c1
- Derived from:
- ccsbBroadEn_13536
- DNA Barcode:
- CATTGACCAAATGTATTGTGCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRG1BP (284802)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100132352 | FRG1HP | FSHD region gene 1 family m... | NR_034006.3 | 17.6% | (many diffs) | |
| 2 | human | 642236 | FRG1JP | FSHD region gene 1 family m... | NR_033907.2 | 15.9% | (many diffs) | |
| 3 | human | 100132352 | FRG1HP | FSHD region gene 1 family m... | NR_156730.1 | 9.3% | (many diffs) | |
| 4 | human | 283788 | LOC283788 | FSHD region gene 1 pseudogene | NR_027436.2 | 6.4% | (many diffs) | |
| 5 | human | 102723390 | FRG1EP | FSHD region gene 1 family m... | NR_146067.1 | 6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 315
- ORF length:
- 249
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA CTCCACCCCC GCGAATCCGG TGGAAGCCGT GGTGCAGAGA GCCGGCTTTG 121 TGGCCTCCCA GGCTTCGCTC CGACCCTGGA AACAGGATAG ACGGGCGGGT GACCCGTGGC 181 CCCGTACCCA CGAGTTTGGG TACCCTGAGG CATCTCTCCA GGCCTCTGCC TGTAAGAAGA 241 AAAAGAGCAA AGATAAGAAG AGAAAAAGAG AAGATGAAGA AACCCAGCTT GATACGTTGG 301 AATCTGGTGA ACAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 361 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 421 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CATTGACCAA ATGTATTGTG 481 CGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt