Construct: ORF TRCN0000465838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015090.1_s317c1
- Derived from:
- ccsbBroadEn_00637
- DNA Barcode:
- ATGGTTCCGCCAAACGTGCTTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBLIF (2694)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2694 | CBLIF | cobalamin binding intrinsic... | NM_005142.3 | 100% | 100% | |
| 2 | human | 2694 | CBLIF | cobalamin binding intrinsic... | XM_011544939.3 | 96.6% | 96.6% | 828_829ins42 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1317
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctggtttgcc ctctacctcc tgagccttct ctgggctaca gctgggacta 121 gtacccagac ccagagttca tgctccgttc cctcagcaca ggagcccttg gtcaatggaa 181 tacaagtact catggagaac tcggtgactt catcagccta cccaaacccc agcatcctga 241 ttgccatgaa tctggccgga gcctacaact tgaaggccca gaagctcctg acttaccagc 301 tcatgtccag cgacaacaac gatctaacca ttgggcagct cggcctcacc atcatggccc 361 tcacctcctc ctgccgagac cctggggata aagtatccat tctacaaaga caaatggaga 421 actgggcacc ttccagcccc aacgctgaag catcagcctt ctatgggccc agtctagcga 481 tcttggcact gtgccagaag aactctgagg cgaccttgcc gatagccgtc cgctttgcca 541 agaccctgct ggccaactcc tctcccttca atgtagacac aggagcaatg gcaaccttgg 601 ctctgacctg tatgtacaac aagatccctg taggttcaga ggaaggttac agatccctgt 661 ttggtcaggt actaaaggat attgtggaga aaatcagcat gaagatcaaa gataatggca 721 tcattggaga catctacagt actggcctcg ccatgcaggc tctctctgta acacctgagc 781 catctaaaaa ggaatggaac tgcaagaaga ctacggatat gatactcaat gagattaagc 841 aggggaaatt ccacaacccc atgtccattg ctcaaatcct cccttcccTG AAAGGCAAGA 901 CATACCTAGA TGTGCCCCAG GTCACTTGTA GTCCTGATCA TGAGGTACAA CCAACTCTAC 961 CCAGCAACCC TGGCCCTGGC CCCACCTCTG CATCTAACAT CACTGTCATA TACACCATAA 1021 ATAACCAGCT GAGGGGGGTT GAGCTGCTCT TCAACGAGAC CATCAATGTT AGTGTGAAAA 1081 GTGGGTCAGT GTTACTTGTT GTCCTAGAGG AAGCACAGCG CAAAAATCCT ATGTTCAAAT 1141 TTGAAACCAC AATGACATCT TGGGGCCTTG TCGTCTCTTC TATCAACAAT ATCGCGGAAA 1201 ATGTTAATCA CAAGACATAC TGGCAGTTTC TTAGTGGTGT AACACCTTTG AATGAAGGGG 1261 TTGCTGACTA CATACCCTTC AACCACGAGC ACATCACAGC CAATTTCACA CAGTACTACC 1321 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1381 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1441 ATATATCTTG TGGAAAGGAC GAATGGTTCC GCCAAACGTG CTTTTCACGC GTTAAGTCga 1501 caatcaacct ctggattaca aaatttgtga aagatt