Construct: ORF TRCN0000465842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002751.1_s317c1
- Derived from:
- ccsbBroadEn_03666
- DNA Barcode:
- GAGGCAGATTAAAGCATGGCATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CISD1 (55847)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465842
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55847 | CISD1 | CDGSH iron sulfur domain 1 | NM_018464.5 | 100% | 100% | |
| 2 | mouse | 52637 | Cisd1 | CDGSH iron sulfur domain 1 | NM_134007.4 | 81.5% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 414
- ORF length:
- 324
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAGTCTGAC TTCCAGTTCC AGCGTACGAG 121 TTGAATGGAT CGCAGCAGTT ACCATTGCTG CTGGGACAGC TGCAATTGGT TATCTAGCTT 181 ACAAAAGATT TTATGTTAAA GATCATCGAA ATAAAGCTAT GATAAACCTT CACATCCAGA 241 AAGACAACCC CAAGATAGTA CATGCTTTTG ACATGGAGGA TTTGGGAGAT AAAGCTGTGT 301 ACTGCCGTTG TTGGAGGTCC AAAAAGTTCC CATTCTGTGA TGGGGCTCAC ACAAAACATA 361 ACGAAGAGAC TGGAGACAAT GTGGGCCCTC TGATCATCAA GAAAAAAGAA ACTTACCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAG AGGCAGATTA AAGCATGGCA TACACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att