Construct: ORF TRCN0000465850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015572.1_s317c1
- Derived from:
- ccsbBroadEn_01317
- DNA Barcode:
- AGTTCACTATCAATGTATGGCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMC5 (5705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5705 | PSMC5 | proteasome 26S subunit, ATP... | NM_002805.6 | 99.9% | 99.7% | 1218G>C |
| 2 | human | 5705 | PSMC5 | proteasome 26S subunit, ATP... | NM_001199163.1 | 97.9% | 97.7% | 0_1ins24;1194G>C |
| 3 | human | 5705 | PSMC5 | proteasome 26S subunit, ATP... | XM_024450840.1 | 93.6% | 93.5% | 322_402del;1299G>C |
| 4 | human | 5705 | PSMC5 | proteasome 26S subunit, ATP... | XM_024450841.1 | 91.8% | 91.6% | 0_1ins24;298_378del;1275G>C |
| 5 | human | 5705 | PSMC5 | proteasome 26S subunit, ATP... | XR_934508.2 | 90.3% | 1_60del;738_753del;1294_1347delinsC | |
| 6 | human | 222161 | DKFZP586I1420 | uncharacterized protein DKF... | NR_002186.1 | 36% | (many diffs) | |
| 7 | mouse | 19184 | Psmc5 | protease (prosome, macropai... | NM_008950.1 | 90.1% | 99.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcttgacgga ccagagcaga tggagctgga ggaggggaag gcaggcagcg 121 gactccgcca atattatctg tccaagattg aagaactcca gctgattgtg aatgataaga 181 gccaaaacct ccggaggctg caggcacaga ggaacgaact aaatgctaaa gttcgcctat 241 tgcgggagga gctacagctg ctgcaggagc agggctccta tgtgggggaa gtagtccggg 301 ccatggataa gaagaaagtg ttggtcaagg tacatcctga aggtaaattt gttgtagacg 361 tggacaaaaa cattgacatc aatgatgtga cacccaattg ccgggtggct ctaaggaatg 421 acagctacac tctgcacaag atcctgccca acaaggtaga cccattagtg tcactgatga 481 tggtggagaa agtaccagat tcaacttatg agatgattgg tggactggac aaacagatca 541 aggagatcaa agaagtgatc gagctgcctg ttaagcatcc tgagctcttc gaagcactgg 601 gcattgctca gcccaaggga gtgctgctgt atggacctcc aggcactggg aagacactgt 661 tggcccgggc tgtggctcat catacggact gtacctttat tcgtgtctct ggctctgaac 721 tggtacagaa attcataggg gaaggggcaa gaatggtgag ggagctgttt gtcatggcac 781 gggaacatgc tccatctatc atcttcatgg acgaaatcga ctccatcggc tcctcgcggc 841 tggagggggg ttctggaggg gacagtgaag tgcagcgcac gatgctGGAG TTGCTCAACC 901 AGCTCGACGG CTTTGAGGCC ACCAAGAACA TCAAGGTTAT CATGGCTACT AATAGGATTG 961 ATATCCTGGA CTCGGCACTG CTTCGCCCAG GGCGCATTGA CAGAAAAATT GAATTCCCAC 1021 CCCCCAATGA GGAGGCCCGG CTGGACATTT TGAAGATTCA TTCTCGGAAG ATGAACCTGA 1081 CCCGGGGGAT CAACCTGAGA AAAATTGCTG AGCTCATGCC AGGAGCATCA GGGGCTGAAG 1141 TGAAGGGCGT GTGCACAGAA GCTGGCATGT ATGCCCTGCG AGAACGGCGA GTCCATGTCA 1201 CTCAGGAGGA CTTTGAGATG GCAGTAGCCA AGGTCATGCA GAAGGACAGT GAGAAAAACA 1261 TGTCCATCAA GAAATTATGG AACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GTTCACTATC 1441 AATGTATGGC TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att