Construct: ORF TRCN0000465851
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004113.1_s317c1
- Derived from:
- ccsbBroadEn_03448
- DNA Barcode:
- CTCTCCCGCTAGCACTGCACCTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MARCHF5 (54708)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465851
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54708 | MARCHF5 | membrane associated ring-CH... | NM_017824.5 | 100% | 100% | |
| 2 | human | 54708 | MARCHF5 | membrane associated ring-CH... | XM_011539886.3 | 72.9% | 71.5% | (many diffs) |
| 3 | mouse | 69104 | March5 | membrane-associated ring fi... | NM_027314.3 | 92.8% | 100% | (many diffs) |
| 4 | mouse | 69104 | March5 | membrane-associated ring fi... | XM_006527322.2 | 88.3% | 95.2% | (many diffs) |
| 5 | mouse | 69104 | March5 | membrane-associated ring fi... | NM_001164336.1 | 80.1% | 86.3% | (many diffs) |
| 6 | mouse | 69104 | March5 | membrane-associated ring fi... | NM_001164337.1 | 48.5% | 46.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 900
- ORF length:
- 834
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggaccaagcc ctacagcaga tgctggacag aagttgctgg gtttgttttg 121 ctactgatga agatgataga acagctgaat gggtgagacc atgcaggtgc agaggatcta 181 caaaatgggt tcaccaggcc tgtctacaac gctgggtgga tgaaaagcaa agaggaaaca 241 gtacagccag agtggcatgt cctcagtgca atgctgaata cctaatagtt tttccaaaat 301 tgggtccagt ggtttacgtc ttggatcttg cagatagact gatctcaaaa gcctgtccat 361 ttgctgcagc aggaataatg gtcggctcta tctattggac agctgtgact tatggagcag 421 tgacagtgat gcaggttgta ggtcataaag aaggtctgga tgttatggag agagctgatc 481 ctttattcct tttaattgga cttcctacta ttcctgtcat gctgatatta ggcaagatga 541 ttcgctggga ggactatgtg cttagactgt ggcgcaaata ctcgaataaa ctacaaattt 601 taaatagtat atttccaggg ataggttgtc ctgttcctcg aattccagct gaggccaatc 661 ctttagcaga tcatgtctct gctactcgaa tcttgtgtgg agcccttgtc tttcctacta 721 ttgctacaat agttggtaaa ttgatgttca gtagtgttaa ctctaattta caaaggacaa 781 tcttgggtgg aattgcgttt gttgccataa aaggagcatt taAAGTTTAC TTCAAACAGC 841 AGCAATATTT ACGACAGGCA CACCGCAAAA TTCTGAATTA TCCAGAACAA GAAGAAGCAT 901 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 961 GTCTCGATTC TACGTAGTAA TGAACTAGCC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1021 TTTATATATC TTGTGGAAAG GACGACTCTC CCGCTAGCAC TGCACCTCAA CGCGTTAAGT 1081 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt