Construct: ORF TRCN0000465854
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013348.3_s317c1
- Derived from:
- ccsbBroadEn_01495
- DNA Barcode:
- GCCATTTCAGTCTTGAGAGTGACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL2 (6347)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465854
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6347 | CCL2 | C-C motif chemokine ligand 2 | NM_002982.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 387
- ORF length:
- 297
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAAGTCTC TGCCGCCCTT CTGTGCCTGC 121 TGCTCATAGC AGCCACCTTC ATTCCCCAAG GGCTCGCTCA GCCAGATGCA ATCAATGCCC 181 CAGTCACCTG CTGTTATAAC TTCACCAATA GGAAGATCTC AGTGCAGAGG CTCGCGAGCT 241 ATAGAAGAAT CACCAGCAGC AAGTGTCCCA AAGAAGCTGT GATCTTCAAG ACCATTGTGG 301 CCAAGGAGAT CTGTGCTGAC CCCAAGCAGA AGTGGGTTCA GGATTCCATG GACCACCTGG 361 ACAAGCAAAC CCAAACTCCG AAGACTTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCATTTC 541 AGTCTTGAGA GTGACGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt