Construct: ORF TRCN0000465855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003498.1_s317c1
- Derived from:
- ccsbBroadEn_14201
- DNA Barcode:
- TCATACGATCATAGCGAAGCCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2Q1 (55585)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465855
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55585 | UBE2Q1 | ubiquitin conjugating enzym... | NM_017582.7 | 16.7% | 16.5% | 1_1053del;1262A>C |
| 2 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_001346658.1 | 22.7% | 25% | (many diffs) |
| 3 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_001346657.1 | 16.9% | 18.6% | (many diffs) |
| 4 | mouse | 70093 | Ube2q1 | ubiquitin-conjugating enzym... | NM_027315.4 | 15.7% | 16.5% | (many diffs) |
| 5 | mouse | 109161 | Ube2q2 | ubiquitin-conjugating enzym... | NM_180600.3 | 15.3% | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 355
- ORF length:
- 213
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGGAACTT CTCACCAAAC AGGGCTGGAG CAGTGCCTAC 181 TCCATAGAGT CAGTGATCAT GCAGATCAGT GCCACACTGG TGAAGGGGAA AGCACGAGTG 241 CAGTTTGGAG CCAACAAATC TCAATACAGT CTGACAAGAG CACAGCAGTC CTACAAGTCC 301 TTGGTGCAGA TCCACGAAAA AAACGGCTGG TACACACCCC CAAAAGAAGC CGGCTACCCA 361 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 421 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 481 ATATCTTGTG GAAAGGACGA TCATACGATC ATAGCGAAGC CTTTACGCGT TAAGTCgaca 541 atcaacctct ggattacaaa atttgtgaaa gatt