Construct: ORF TRCN0000465855

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003498.1_s317c1
Derived from:
ccsbBroadEn_14201
DNA Barcode:
TCATACGATCATAGCGAAGCCTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UBE2Q1 (55585)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465855
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55585 UBE2Q1 ubiquitin conjugating enzym... NM_017582.7 16.7% 16.5% 1_1053del;1262A>C
2 mouse 109161 Ube2q2 ubiquitin-conjugating enzym... NM_001346658.1 22.7% 25% (many diffs)
3 mouse 109161 Ube2q2 ubiquitin-conjugating enzym... NM_001346657.1 16.9% 18.6% (many diffs)
4 mouse 70093 Ube2q1 ubiquitin-conjugating enzym... NM_027315.4 15.7% 16.5% (many diffs)
5 mouse 109161 Ube2q2 ubiquitin-conjugating enzym... NM_180600.3 15.3% 16.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
142
ORF end:
355
ORF length:
213
Sequence:
1TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG
61TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA
121AGTTTGTACA AAAAAGTTGG CATGGAACTT CTCACCAAAC AGGGCTGGAG CAGTGCCTAC
181TCCATAGAGT CAGTGATCAT GCAGATCAGT GCCACACTGG TGAAGGGGAA AGCACGAGTG
241CAGTTTGGAG CCAACAAATC TCAATACAGT CTGACAAGAG CACAGCAGTC CTACAAGTCC
301TTGGTGCAGA TCCACGAAAA AAACGGCTGG TACACACCCC CAAAAGAAGC CGGCTACCCA
361ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC
421GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT
481ATATCTTGTG GAAAGGACGA TCATACGATC ATAGCGAAGC CTTTACGCGT TAAGTCgaca
541atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)