Construct: ORF TRCN0000465856
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003974.1_s317c1
- Derived from:
- ccsbBroadEn_09623
- DNA Barcode:
- AGGATCTGTGCCCGTCGTTCTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ISM2 (145501)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465856
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145501 | ISM2 | isthmin 2 | NM_199296.3 | 99.9% | 99.8% | 280G>A |
| 2 | human | 145501 | ISM2 | isthmin 2 | XM_011536489.2 | 84.5% | 84.4% | 0_1ins264;16G>A |
| 3 | human | 145501 | ISM2 | isthmin 2 | NM_182509.4 | 51% | 35.9% | 280G>A;626_627ins346;876_877ins491 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1782
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgtgcgctc cgcgaccgag ccgggctcct cctctgcgtg ctgctgctgg 121 cggcgctgct ggaggcggcg ctagggctcc ccgtgaagaa gccgcggctc cgcggaccac 181 ggcctgggag cctcacgagg ctcgcagagg tctcagcctc cccagatcct aggcctctga 241 aggaagagga ggaggcacca ctgctcccca gaacccacct gcaggcagag ccacaccaac 301 atggatgctg gactgtcact gagccagcag ccatgacccc aggcaacacc acccctccca 361 ggaccccaga ggttactccg ttgcggctgg agctgcagaa gctgccggga ttggccaaca 421 caaccttgag tacccctaac cctgataccc aggcttcagc ctccccagat cctaggcctc 481 tgagggaaga ggaggaggca cgactgctcc ccagaaccca cctgcaggca gagctacacc 541 aacatggatg ttggactgtc actgagccag cagccctgac cccagggaat gccacgcctc 601 ccaggaccca ggaggttact cccttgctgc tggagctgca gaagctgcca gaattggtcc 661 acgcaacctt gagtacccct aaccctgata accaggtgac catcaaggtg gtggaggacc 721 cccaggccga ggtgtcgata gacctgttgg ctgagcccag caatcccccg ccccaggata 781 cccttagctg gctgcccgcc ctctggtcct tcctctgggg agactacaaa ggagaggaaa 841 aagacagggc cccaggggag aagggggagg aaaaggagga agacgaggac tatccttcag 901 aggatatcga gggtgaggat caagaggaca aagaggaaga tgaggaagag caggcgctct 961 ggttcaatgg aactacagac aactgggacc agggctggct ggcccccggg gattgggtct 1021 tcaaggattc tgtcagctac gactatgagc ctcagaagga gtggagtccc tggtctccct 1081 gcagtgggaa ctgcagcact ggcaagcagc agaggactcg gccctgtggc tatggctgca 1141 ctgccaccga gacccgtacc tgtgacctgc cctcctgtcc tggcactgag gacaaggaca 1201 ccttgggcct ccccagtgag gagtggaagc tcctggcccg caatgctacg gacatgcatg 1261 atcaagatgt ggacagctgt gagaagtggc tgaactgcaa gagcgacttc ctaatcaagt 1321 atctgagcca gatgctgcgg gacctgccca gctgcccgtg tgcctaccca ctggaggcca 1381 tggacagccc tgtgagccTA CAGGACGAGC ACCAGGGCCG CAGCTTCCGG TGGAGGGATG 1441 CCAGTGGCCC TCGCGAGCGC CTGGACATCT ACCAGCCCAC GGCGCGCTTC TGCCTGCGTT 1501 CCATGCTGTC TGGGGAGAGC AGCACACTGG CCGCCCAGCA CTGCTGCTAT GACGAGGACA 1561 GCCGGCTGCT GACCCGTGGC AAGGGCGCCG GCATGCCCAA CCTCATCAGC ACCGACTTCT 1621 CACCTAAGCT GCACTTCAAG TTCGACACGA CGCCCTGGAT CCTGTGCAAG GGGGACTGGA 1681 GCCGCCTCCA CGCTGTGCTC CCTCCCAACA ACGGCCGAGC CTGCACCGAC AACCCCCTGG 1741 AGGAGGAGTA CCTAGCACAG TTGCAGGAGG CCAAGGAGTA CTTGCCAACT TTCTTGTACA 1801 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1861 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1921 AGGACGAAGG ATCTGTGCCC GTCGTTCTCT CACGCGTTAA GTCgacaatc aacctctgga 1981 ttacaaaatt tgtgaaagat t