Construct: ORF TRCN0000465859
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001997.1_s317c1
- Derived from:
- ccsbBroadEn_04637
- DNA Barcode:
- CCTGATTCTTCAATATCCATGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KTI12 (112970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465859
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112970 | KTI12 | KTI12 chromatin associated ... | NM_138417.3 | 100% | 100% | |
| 2 | human | 51060 | TXNDC12 | thioredoxin domain containi... | NR_046406.1 | 34.6% | 1_1400del;2463_3065del | |
| 3 | human | 51060 | TXNDC12 | thioredoxin domain containi... | NR_046405.1 | 33.3% | 1_1523del;2586_3188del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc gctcgtggtg ttttgcgggc tgccgtacag cggcaagagc cggcgtgctg 121 aagagttgcg cgtggcgctg gctgccgagg gccgcgcggt gtacgtggtg gacgacgcag 181 ctgtcctggg cgcagaggac ccagcggtgt acggcgattc tgcccgtgag aaggcattgc 241 gtggagctct gcgagcctcc gtggaacggc gcctgagtcg ccacgacgtg gtcatcctgg 301 actcgcttaa ctacatcaaa ggtttccgtt acgagctcta ctgcctggca cgggcggcgc 361 gcaccccgct ctgcctggtc tactgcgtac ggcccggcgg cccgatcgcg ggacctcagg 421 tggcgggcgc gaacgagaac cctggccgga acgtcagtgt gagttggcgg ccacgcgctg 481 aggaggacgg gagagcccag gcggcgggca gcagcgtcct cagggaactg catactgcgg 541 actctgtagt aaatggaagt gcccaggccg acgtacccaa ggaactggag cgagaagaat 601 ccggggctgc ggagtctcca gctcttgtga ctccggattc agagaaatct gcaaagcatg 661 ggtccggtgc cttttactct cccgaactcc tggaggccct aacgctgcgc tttgaggctc 721 ccgattctcg gaatcgctgg gaccggcctt tattcacttt ggtgggccta gaggagccgt 781 tgcccctggc ggggatccgc tctgccctgt ttgagaaccg ggccccacca ccccatcagt 841 ctacgcagtc ccagcccctc gccTCCGGCA GCTTTCTGCA CCAGTTGGAC CAGGTCACGA 901 GTCAAGTACT GGCCGGATTG ATGGAAGCGC AGAAGAGCGC TGTCCCCGGG GACTTGCTCA 961 CGCTTCCTGG TACCACAGAG CACTTGCGGT TTACCCGGCC CTTGACCATG GCAGAACTGA 1021 GTCGCCTTCG TCGCCAGTTT ATTTCGTACA CTAAAATGCA TCCCAACAAT GAGAACTTGC 1081 CGCAACTGGC CAACATGTTT CTTCAGTATT TGAGCCAGAG CCTGCACTGC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGACCTGATT CTTCAATATC CATGTATACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt