Construct: ORF TRCN0000465882
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015054.1_s317c1
- Derived from:
- ccsbBroadEn_13016
- DNA Barcode:
- AAGGATTACATCCCCCTCATGGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AHNAK2 (113146)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465882
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113146 | AHNAK2 | AHNAK nucleoprotein 2 | NM_001350929.2 | 8.4% | 8.4% | 1_15636del;15889C>G |
| 2 | human | 113146 | AHNAK2 | AHNAK nucleoprotein 2 | XM_024449463.1 | 8.4% | 8.4% | 1_15636del;15889C>G |
| 3 | human | 113146 | AHNAK2 | AHNAK nucleoprotein 2 | NM_138420.4 | 8.3% | 8.3% | 1_15936del;16189C>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1515
- ORF length:
- 1449
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcttccagaa acccaggttc ttccaggaga aatagatgag actcctcttt 121 ccaagccagg acatgacctt gccagcatgg aggataaaac agagaaatgg tcttcccagc 181 ctgaaggtcc acttaaattg aaagcttcaa gtactgatat gccatcccag atttctgtgg 241 ttaatgtgga tcaactgtgg gaagattctg tcctaactgt caaattcccc aaattaatgg 301 taccaaggtt ctccttcgct gcccccagct cagaggatga tgtgttcatc cccactgtga 361 gggaagtgca gtgtccagag gccaatattg atacagccct ttgtaaggaa agtccggggc 421 tctggggagc cagcatcctg aaggcaggtg ctggggtccc tggggagcag cctgtggacc 481 ttaacctgcc tttggaagct cccccaattt caaaggtcag agtgcatatt cagggtgctc 541 aggttgaaag tcaagaggtc actatacaca gcatagtgac accagagttt gtagatctct 601 cagtacccag gactttttcc actcagattg tgcgggaatc agagatcccc acgtcagaga 661 ttcaaacacc ttcgtacgga ttttccttat taaaagtgaa aatcccagag ccccacacgc 721 aggctagagt gtacacaaca atgactcaac actctaggac tcaggagggc acagaagagg 781 ctcccataca agccacccca ggagtagact ccatttctgg agatctccag cctgacactg 841 gagaaccatt tgagatgatc tcttccagcg tcaatgtact gggacagcaa acactcacat 901 ttgaagttcc ttctggccac cagcttgcag acagctgttc agatgaggag ccagcagaaa 961 ttcttgagtt tccccctgat gatagccaag aggcaaccac accactggca gatgaaggca 1021 gggctccaaa agacaaacca gaaagtaaaa aatctggtct gctctggttt tggcttccaa 1081 acattgggtt ttcctcttct gttgatgaga caggtgttga ttccaaaaat gacgtccaga 1141 gatctgctcc cattcaaaca cagccTGAGG CACGACCAGA GGCAGAACTG CCTAAAAAAC 1201 AGGAGAAGGC AGGCTGGTTC CGATTTCCCA AATTAGGGTT CTCCTCATCT CCTACCAAGA 1261 AAAGCAAAAG CACCGAAGAT GGGGCAGAGC TGGAAGAACA AAAACTTCAA GAAGAAACAA 1321 TCACGTTTTT TGATGCCCGA GAAAGTTTCT CCCCTGAAGA GAAGGAAGAG GGTGAACTGA 1381 TCGGGCCTGT GGGCACTGGG CTGGACTCCA GAGTGATGGT GACATCCGCG GCAAGAACAG 1441 AGTTAATCCT GCCCGAGCAG GACAGAAAAG CTGACGATGA AAGCAAAGGG TCAGGCCTGG 1501 GACCAAATGA AGGCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1561 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1621 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AAGGATTACA TCCCCCTCAT 1681 GGGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt