Construct: ORF TRCN0000465889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010857.1_s317c1
- Derived from:
- ccsbBroadEn_06230
- DNA Barcode:
- AGCGACACACGAAATTTACTGCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FUT2 (2524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2524 | FUT2 | fucosyltransferase 2 | NM_000511.6 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 2524 | FUT2 | fucosyltransferase 2 | NM_001097638.3 | 99.6% | 99.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1098
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctggtcgtt cagatgcctt tctcctttcc catggcccac ttcatcctct 121 ttgtctttac ggtttccact atatttcacg ttcagcagcg gctancgaag attcaagcca 181 tgtgggagtt accggtgcag ataccagtgc tagcctcaac atcaaaggca ctgggaccca 241 gccagctcag ggggatgtgg acgatcaatg caataggccg cctggggaac cagatgggcg 301 agtacgccac actgtacgcc ctggccaaga tgaacgggcg gcccgccttc atcccggccc 361 agatgcacag caccctggcc cccatcttca gaatcaccct gccggtgctg cacagcgcca 421 cggccagcag gatcccctgg cagaactacc acctgaatga ctggatggag gaggaatacc 481 gccacttccc tggggagtac gtccgcttca ccggctaccc ctgctcctgg accttctacc 541 accacctccg ccaggagatc ctccaggagt tcaccctgca cgaccacgtg cgggaggagg 601 cccagaagtt cctgcggggc ctgcaggtga acgggagccg gccgggcacc tttgtagggg 661 tccatgttcg ccgaggggac tatgtccatg tcatgccaaa agtgtggaag ggggtggtgg 721 ccgaccggcg atacctacag caggccctgg actggttccg agctcgctac agctccctca 781 tcttcgtggt caccagtaat ggcatggcct ggtgtcggga gaacattgac accTCCCACG 841 GTGATGTGGT GTTTGCTGGC GATGGCATTG AGGGCTCACC TGCCAAAGAT TTTGCTCTAC 901 TCACACAGTG TAACCACACC ATCATGACCA TTGGGACGTT CGGGATCTGG GCCGCATACC 961 TCACGGGCGG AGACACCATC TACCTGGCCA ATTACACCCT CCCCGACTCC CCTTTCCTCA 1021 AAATCTTTAA GCCAGAGGCA GCCTTCCTGC CGGAGTGGAC AGGGATTGCC GCAGACCTGT 1081 CCCCCTTACT CAAGCACTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1141 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1201 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAGCGACA CACGAAATTT 1261 ACTGCAGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt