Transcript: Human NM_000511.6

Homo sapiens fucosyltransferase 2 (FUT2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
FUT2 (2524)
Length:
3116
CDS:
122..1153

Additional Resources:

NCBI RefSeq record:
NM_000511.6
NBCI Gene record:
FUT2 (2524)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000511.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418531 TTTACGGTTTCCACTATATTT pLKO_005 179 CDS 100% 15.000 21.000 N FUT2 n/a
2 TRCN0000431129 CCCTTACTCAAGCACTAATGC pLKO_005 1136 CDS 100% 4.950 6.930 N FUT2 n/a
3 TRCN0000414186 GTGCTAGCCTCAACATCAAAG pLKO_005 260 CDS 100% 10.800 8.640 N FUT2 n/a
4 TRCN0000418672 TGGGTTCAAAGACCATAATCA pLKO_005 1626 3UTR 100% 5.625 3.938 N FUT2 n/a
5 TRCN0000036101 TGCTCTACTCACACAGTGTAA pLKO.1 946 CDS 100% 4.950 3.465 N FUT2 n/a
6 TRCN0000036102 CTATGTCCATGTCATGCCAAA pLKO.1 733 CDS 100% 4.050 2.835 N FUT2 n/a
7 TRCN0000036103 CTGGCAGAACTACCACCTGAA pLKO.1 490 CDS 100% 4.050 2.025 Y FUT2 n/a
8 TRCN0000140719 GATCACTTGAGGTCAGGAGTT pLKO.1 1363 3UTR 100% 4.050 2.025 Y P3H4 n/a
9 TRCN0000165299 GATCACTTGAGGTCAGGAGTT pLKO.1 1363 3UTR 100% 4.050 2.025 Y ORAI2 n/a
10 TRCN0000352971 GATCACTTGAGGTCAGGAGTT pLKO_005 1363 3UTR 100% 4.050 2.025 Y P3H4 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 2531 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 2531 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000511.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06230 pDONR223 100% 99.6% 99.4% None (many diffs) n/a
2 ccsbBroad304_06230 pLX_304 0% 99.6% 99.4% V5 (many diffs) n/a
3 TRCN0000465889 AGCGACACACGAAATTTACTGCAG pLX_317 26.1% 99.6% 99.4% V5 (many diffs) n/a
4 ccsbBroadEn_13859 pDONR223 100% 44.2% 44% None (many diffs) n/a
5 ccsbBroad304_13859 pLX_304 0% 44.2% 44% V5 (many diffs) n/a
6 TRCN0000471573 TCGTCGCGCTCTGCGGGCTGATTA pLX_317 83.9% 44.2% 44% V5 (many diffs) n/a
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