Construct: ORF TRCN0000465891
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003278.1_s317c1
- Derived from:
- ccsbBroadEn_02742
- DNA Barcode:
- AAGTTGCACTGGAGATTTACGACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KAZN (23254)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465891
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001017999.2 | 100% | 100% | |
| 2 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001018001.2 | 100% | 100% | |
| 3 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001370229.1 | 79.3% | 79.3% | 1_255del |
| 4 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001018000.3 | 78.7% | 78.7% | 1_264del |
| 5 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001370231.1 | 77.8% | 77.8% | 1_279del |
| 6 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_015209.2 | 77.6% | 77.6% | 1_282del |
| 7 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_017000771.1 | 77.6% | 77.6% | 1_282del |
| 8 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_001370230.1 | 77.2% | 71.3% | 1_87del;847_848insGCAAC;912_913ins151 |
| 9 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_017000768.2 | 55.4% | 52.6% | (many diffs) |
| 10 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_017000769.2 | 53.3% | 49.8% | (many diffs) |
| 11 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_017000770.2 | 52.9% | 49% | 1_576del;1336_1337insGCAAC;1401_1402ins151 |
| 12 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541080.3 | 52.1% | 50.4% | (many diffs) |
| 13 | human | 23254 | KAZN | kazrin, periplakin interact... | NM_201628.3 | 41.4% | 40.1% | (many diffs) |
| 14 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541076.2 | 40.8% | 39.5% | (many diffs) |
| 15 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541077.2 | 40.8% | 39.5% | (many diffs) |
| 16 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_005245795.5 | 36.7% | 35.6% | (many diffs) |
| 17 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541075.2 | 36.7% | 35.5% | (many diffs) |
| 18 | human | 23254 | KAZN | kazrin, periplakin interact... | XM_011541074.3 | 32.8% | 31.8% | (many diffs) |
| 19 | mouse | 71529 | Kazn | kazrin, periplakin interact... | NM_001109685.1 | 72.3% | 77.5% | (many diffs) |
| 20 | mouse | 71529 | Kazn | kazrin, periplakin interact... | NM_001109684.1 | 70.5% | 75.5% | (many diffs) |
| 21 | mouse | 71529 | Kazn | kazrin, periplakin interact... | NM_144531.3 | 38% | 39.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggagatgttg gcgaaggacc tggaggagtc gcagggcggc aagtcctctg 121 aggtcctctc ggccaccgag ctcagggtcc agctggccca gaaggagcag gagctagcca 181 gagccaaaga agccttgcag gccatgaaag ctgatcggaa gcgcttaaag ggcgagaaga 241 cagacctggt gagccagatg cagcagctgt atgccacact ggagagccgc gaggagcagc 301 tccgagactt catccgcaac tatgagcagc accgcaagga gagcgaggat gcggtcaaag 361 cgctggccaa ggagaaggac ctgctggagc gtgagaagtg ggagctgcgg cgccaagcca 421 aggaggccac agaccacgcc acggcactgc gctcccagct ggacctcaag gacaaccgga 481 tgaaggagct ggaggccgag ctggccatgg ccaaacagtc cttagctacg ctgaccaagg 541 acgtccccaa gcggcattcc ctcgccatgc cgggcgagac ggtgctcaat ggcaaccagg 601 agtgggtggt gcaggcggac ctcccgctga ccgcagccat ccggcagagt caacagactc 661 tctaccactc acacccccct caccctgcgg accggcaagc ggtcagggtg agcccctgcc 721 actcccggca gccctctgtc atcTCCGACG CATCTGCCGC CGAAGGCGAC CGGTCGTCCA 781 CACCGAGCGA CATCAACTCC CCTCGACACC GGACACACTC CCTCTGCAAC GGCGACAGTC 841 CCGGCCCAGT TCAGAAGAAC CTGCACAACC CTATTGTACA GTCACTAGAG GATCTTGAAG 901 ACCAAAAACG GAAAAAGAAG AAAGAGAAGA TGGGATTCGG CTCCATCTCC CGCGTCTTCG 961 CCAGAGGGAA GCAGCGGAAG TCCCTCGACC CCGGCCTCTT TGATGGTACC GCCCCTGATT 1021 ATTACATAGA GGAGGACGCG GACTGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAAGTTGCA 1201 CTGGAGATTT ACGACAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt