Transcript: Human XM_005245795.5

PREDICTED: Homo sapiens kazrin, periplakin interacting protein (KAZN), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KAZN (23254)
Length:
6271
CDS:
242..2863

Additional Resources:

NCBI RefSeq record:
XM_005245795.5
NBCI Gene record:
KAZN (23254)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005245795.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000153449 CATGATGACTATGGCTCTCTT pLKO.1 2750 CDS 100% 4.950 6.930 N KAZN n/a
2 TRCN0000164593 CCCTATTGTACAGTCACTAGA pLKO.1 1621 CDS 100% 4.950 6.930 N KAZN n/a
3 TRCN0000158072 CAGCAGCAAAGATCCCGATTT pLKO.1 2728 CDS 100% 10.800 8.640 N KAZN n/a
4 TRCN0000157902 CTGAACGTGTCCAAGAAGTTC pLKO.1 2234 CDS 100% 4.950 3.960 N KAZN n/a
5 TRCN0000163756 GTACAGTCACTAGAGGATCTT pLKO.1 1628 CDS 100% 4.950 3.960 N KAZN n/a
6 TRCN0000164683 CGAGACTTCATCCGCAACTAT pLKO.1 1055 CDS 100% 5.625 3.938 N KAZN n/a
7 TRCN0000153509 CCCGATTTCCATGATGACTAT pLKO.1 2741 CDS 100% 4.950 3.465 N KAZN n/a
8 TRCN0000157234 GCAGAGAGACTCAAGCTGATT pLKO.1 5392 3UTR 100% 4.950 3.465 N KAZN n/a
9 TRCN0000156818 GCTGCTGTACCAAGTGAACTT pLKO.1 2284 CDS 100% 4.950 3.465 N KAZN n/a
10 TRCN0000165510 GAAGAACCTGCACAACCCTAT pLKO.1 1606 CDS 100% 4.050 2.835 N KAZN n/a
11 TRCN0000156974 GCCTCAGTTCATTGACCAGAT pLKO.1 4299 3UTR 100% 4.050 2.835 N KAZN n/a
12 TRCN0000153885 CGTGAGGATTAACACTTGCTA pLKO.1 4033 3UTR 100% 3.000 2.100 N KAZN n/a
13 TRCN0000164495 CAACCCTATTGTACAGTCACT pLKO.1 1618 CDS 100% 2.640 1.848 N KAZN n/a
14 TRCN0000166808 CAGTTCAGAAGAACCTGCACA pLKO.1 1599 CDS 100% 2.640 1.848 N KAZN n/a
15 TRCN0000158332 CCATGATGACTATGGCTCTCT pLKO.1 2749 CDS 100% 2.640 1.848 N KAZN n/a
16 TRCN0000165571 GTCAACAGACTCTCTACCACT pLKO.1 1401 CDS 100% 2.640 1.848 N KAZN n/a
17 TRCN0000164924 GCAGAGTCAACAGACTCTCTA pLKO.1 1396 CDS 100% 0.495 0.347 N KAZN n/a
18 TRCN0000157507 GCTGGAGACAAGTGGACAATT pLKO.1 5060 3UTR 100% 13.200 7.920 N KAZN n/a
19 TRCN0000165645 GAGTCAACAGACTCTCTACCA pLKO.1 1399 CDS 100% 0.264 0.158 N KAZN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005245795.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02742 pDONR223 100% 36.7% 35.6% None (many diffs) n/a
2 ccsbBroad304_02742 pLX_304 0% 36.7% 35.6% V5 (many diffs) n/a
3 TRCN0000465891 AAGTTGCACTGGAGATTTACGACA pLX_317 31.3% 36.7% 35.6% V5 (many diffs) n/a
4 ccsbBroadEn_11705 pDONR223 100% 25.3% 25.3% None 1_1953del;2410G>A n/a
5 ccsbBroad304_11705 pLX_304 0% 25.3% 25.3% V5 1_1953del;2410G>A n/a
6 TRCN0000466369 CAAGTAGTGCTATACTTATTTTTT pLX_317 48.9% 25.3% 25.3% V5 1_1953del;2410G>A n/a
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