Construct: ORF TRCN0000465900
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004467.1_s317c1
- Derived from:
- ccsbBroadEn_16025
- DNA Barcode:
- ACCTAGCCAACGACGCGGATCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TTLL2 (83887)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465900
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83887 | TTLL2 | tubulin tyrosine ligase like 2 | NM_031949.5 | 99.7% | 99.6% | (many diffs) |
| 2 | human | 83887 | TTLL2 | tubulin tyrosine ligase like 2 | XM_006715572.4 | 87.4% | 87.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1842
- ORF length:
- 1776
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agggcgggac ctgtgttcct ccacacaaag ccaggcgctg ggatctttga 121 gaaccaccac cccagccttt acccttaaca ttccatccga ggcaaaccac actgagcagc 181 cgcctgcagg cctgggagca aggctacagg aagcaggtgt ttccatccct cccaggcgag 241 gccgcccaac accaacactg gagaagaaga aaaaacctca tttgatggcg gaagatgaac 301 cttcaggggc cctcttgaag ccgctggttt ttcgcgttga cgagaccacc ccggctgtgg 361 tgcaaagtgt cctcctggag agggggtgga ataagtttga taagcaggag cagaacgcgg 421 aggactggaa cctgtactgg agggcatcct ctttccgaat gaccgaacac aacagtgtta 481 aaccgtggca gcagctaaac caccaccctg gaaccaccaa gcttaccagg aaagactgtt 541 tggccaaaca cctgaagcac atgaggagga tgtatggcac ttccctgtac cagttcatcc 601 ccctgacgtt cgtcatgccc aatgactata ccaagttcgt ggctgaatac tttcaggaga 661 ggcagatgct gggcaccaag catagctatt ggatttgcaa gcctgctgag ttatctcgtg 721 ggagggggat actaattttc agtgacttta aagacttcat ctttgatgat atgtacatag 781 tgcagaaata tatctccaat cctttactta ttggcagata taaatgtgat ctccgcatct 841 atgtttgtgt tactggcttt aagcctttga ccatttatgt ttatcaggaa gggttggttc 901 ggtttgccac ggaaaagttt gacctcagta atttgcaaaa caattatgcc catttgacca 961 acagcagcat caataaatcc ggggcctctt atgagaagat caaagaagtg attggtcatg 1021 gttgtaaatg gacgctcagc agattttttt cctaccttcg tagctgggat gtggacgatc 1081 tgcttttgtg gaagaaaatc caccgcatgg ttattctcac cattctcgcc attgcaccat 1141 ctgtcccctt tgctgccaat tgctttgagc tctttgggtt tgatattttg attgatgaca 1201 acttgaaacc atggctttta gaggtcaact acagcccagc cttgaccttg gattgttcaa 1261 cagatgtgtt ggtgaagaga aaacttgtcc atgatattat tgacctgatt tacttaaatg 1321 gtctaagaaa tgagggggga gaagccagta atgccacaca tggaaattcc aacatcgatg 1381 ctgcaaaaag tgacagaggt gggcttgatg ctcctgactg tcttccttat gattctcttt 1441 cgttcacaag cagaatgtac aacgaggatg actctgtggt ggagaaagct gtgagtgtgc 1501 gtcctgaagc tgcacctgcc tcccagctgg aaggagagat gagtgggcag gattttcatc 1561 tgtcaacaag ggagatgcca caaagcaagc ccaagttacg gagcaggcac acgcctcaca 1621 agacactcat gcccTACGCG TCCCTCTTCC AGTCGCACTC CTGCAAGACC AAGACCTCCC 1681 CGTGTGTCCT GTCAGACCGT GGCAAAGCTC CAGATCCCCA AGCAGGCAAC TTTGTTCTTG 1741 TTTTTCCTTT CAATGAAGCA ACTCTCGGAG CTTCCAGGAA TGGATTAAAT GTCAAAAGAA 1801 TAATCCAAGA GCTCCAGAAA CTAATGAATA AGCAACATTC CTACCCAACT TTCTTGTACA 1861 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1921 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1981 AGGACGAACC TAGCCAACGA CGCGGATCGT TACGCGTTAA GTCgacaatc aacctctgga 2041 ttacaaaatt tgtgaaagat t